van Holde K E, Miller K, Schabtach E, Libertini L
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.
J Mol Biol. 1991 Jan 20;217(2):307-21. doi: 10.1016/0022-2836(91)90545-h.
The kinetics of association of Octopus dofleini hemocyanin subunits to form the native decameric molecule have been studied with a combination of sedimentation, light scattering and electron microscopy. The reaction, initiated by addition of magnesium, is relatively slow, requiring hours to reach completion, with monomer and decamer as predominant molecular species throughout. Analysis of the light-scattering data, including stopped-flow studies, reveals an initial lag period in the reaction, followed by a second-order process that is rate limiting. The lag period depends on both protein and magnesium ion concentration. Electron microscope studies reveal intermediates in the process, and support a model of assembly in which nucleation begins at the dimer level. Theoretical models for the process are compared.
利用沉降、光散射和电子显微镜相结合的方法，研究了北太平洋巨型章鱼血蓝蛋白亚基缔合形成天然十聚体分子的动力学。由添加镁引发的反应相对较慢，需要数小时才能完成，在此过程中单体和十聚体是主要的分子种类。对光散射数据的分析，包括停流研究，揭示了反应中最初的延迟期，随后是一个限速的二级过程。延迟期取决于蛋白质和镁离子的浓度。电子显微镜研究揭示了该过程中的中间体，并支持了一种组装模型，即成核始于二聚体水平。对该过程的理论模型进行了比较。
