Miyamaru Satoru, Kumai Yoshihiko, Ito Takaaki, Sanuki Tetsuji, Yumoto Eiji
Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kumamoto University, Kumamoto, Japan.
Acta Otolaryngol. 2009 Dec;129(12):1486-92. doi: 10.3109/00016480902814280.
Nerve-muscle pedicle (NMP) implantation was effective in the recovery from atrophic changes in long-term denervated thyroarytenoid (TA) muscle. Re-innervation occurred via the transferred nerve. However, the effectiveness of the NMP method may decline with increasing duration of denervation.
To evaluate the effects of NMP implantation on long-term denervated rat TA muscle.
Wistar rats (n=105) were divided into two groups in which the left recurrent laryngeal nerve (RLN) was transected without (DNV group) or with (NMP group) subsequent NMP implantation, and subgroups of each group were formed depending on the period after RLN transection (immediate to 48 weeks). In the DNV subgroups, we histologically assessed the area of muscle and the number of neuromuscular junctions. In the NMP subgroups, we performed electromyographic, videolaryngoscopic, and histologic assessments. The muscle area and muscle action potentials were evaluated by comparing the treated and untreated sides. The ratio of the number of nerve terminals to that of acetylcholine receptors was also assessed.
The TA muscle area was significantly larger in most of the NMP subgroups compared with the DNV subgroups. Muscle action potentials were present in all NMP animals. All histologic and physiologic assessments revealed degradation as the denervation period in the five NMP subgroups.
神经肌肉蒂（NMP）植入术对长期失神经支配的甲杓肌（TA）萎缩性改变的恢复有效。再支配通过转移的神经发生。然而，NMP方法的有效性可能会随着失神经支配时间的延长而下降。
评估NMP植入术对长期失神经支配的大鼠TA肌的影响。
将105只Wistar大鼠分为两组，其中一组切断左侧喉返神经（RLN）但不进行后续NMP植入（DNV组），另一组切断左侧喉返神经后进行NMP植入（NMP组），每组再根据RLN切断后的时间（即刻至48周）分为亚组。在DNV亚组中，我们对肌肉面积和神经肌肉接头数量进行了组织学评估。在NMP亚组中，我们进行了肌电图、视频喉镜和组织学评估。通过比较治疗侧和未治疗侧来评估肌肉面积和肌肉动作电位。还评估了神经末梢数量与乙酰胆碱受体数量的比值。
与DNV亚组相比，大多数NMP亚组的TA肌面积明显更大。所有NMP动物均存在肌肉动作电位。所有组织学和生理学评估均显示，五个NMP亚组中的失神经支配期出现了退化。
