Miyamaru Satoru, Kumai Yoshihiko, Minoda Ryosei, Yumoto Eiji
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kumamoto University, 1-1-1 Honjo, Kumamoto, Japan.
Acta Otolaryngol. 2012 Feb;132(2):210-7. doi: 10.3109/00016489.2011.630018. Epub 2011 Nov 21.
Nerve-muscle pedicle (NMP) implantation was effective in the recovery of atrophic changes in the denervated thyroarytenoid (TA) muscle in aged rats.
To evaluate the effects of NMP implantation on the denervated TA muscle in aged rats.
Wistar rats aged 20 months and 8 weeks were respectively divided into two groups in which the left recurrent laryngeal nerve (RLN) was transected without (aged/young DNV group) or with (aged/young NMP group) NMP implantation. The aged DNV and NMP groups were further divided into two subgroups, based on the period after RLN transection (10 or 20 weeks). In the DNV groups, we assessed the area of muscle and the number of neuromuscular junctions (NMJs) histologically. In the NMP groups, we performed electromyography and histological assessments. For electromyography, we stimulated the transferred nerve and evaluated the muscle action potentials (MAPs) of the TA muscle.
The muscle areas in the aged NMP groups were significantly larger than those in the aged DNV groups. More NMJs were found in aged NMP groups compared with aged DNV groups. MAPs were seen in all NMP animals. No significant differences were observed between the aged and young NMP groups in histological and physiological assessments.
神经肌肉蒂（NMP）植入对老年大鼠失神经支配的甲杓肌（TA）萎缩性变化的恢复有效。
评估NMP植入对老年大鼠失神经支配的TA肌的影响。
将20个月龄和8周龄的Wistar大鼠分别分为两组，切断左侧喉返神经（RLN），其中一组不进行NMP植入（老年/年轻去神经支配组），另一组进行NMP植入（老年/年轻NMP组）。根据RLN切断后的时间（10周或20周），将老年去神经支配组和NMP组进一步分为两个亚组。在去神经支配组中，我们通过组织学方法评估肌肉面积和神经肌肉接头（NMJ）的数量。在NMP组中，我们进行了肌电图和组织学评估。对于肌电图，我们刺激转移的神经并评估TA肌的肌肉动作电位（MAP）。
老年NMP组的肌肉面积明显大于老年去神经支配组。与老年去神经支配组相比，老年NMP组发现更多的NMJ。所有NMP动物均可见MAP。在组织学和生理学评估中，老年和年轻NMP组之间未观察到显著差异。
