Li Qian, Huang Chengzhi, Huang Yuming
Department of Chemistry and Chemical Engineering, Institute of Analytical Sciences, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Southwest Normal University, Beibei, Chongqing 400715, People's Republic of China.
Luminescence. 2006 Jan-Feb;21(1):43-8. doi: 10.1002/bio.881.
It was found that light emission produced by the oxidation of luminol by potassium ferricyanide in basic medium was enhanced by synephrine, an anti-obesity drug. The optimum conditions for this chemiluminescent reaction were studied in detail in a flow injection system and employed in a new, simple and rapid method for the determination of synephrine. A mechanism for this reaction is proposed, based on the chemiluminescence reaction spectra. In the optimum conditions, CL intensity is proportional to concentration of synephrine in the 0.008-1 microg/mL range. The limit of detection is 1.6 ng/mL for synephrine (3sigma), and the relative standard deviation (n = 11) is 2.6% for 0.5 microg/mL synephrine. The method was applied to the determination of synephrine in herbal products, citrus fruit and biological fluids. The recoveries were satisfactory (90-102%). The results given by the proposed method are in good agreement with those given by HPLC-UV.
研究发现，在碱性介质中，抗肥胖药物辛弗林可增强铁氰化钾氧化鲁米诺产生的发光。在流动注射系统中详细研究了该化学发光反应的最佳条件，并将其应用于一种测定辛弗林的新的、简单快速的方法中。基于化学发光反应光谱提出了该反应的机理。在最佳条件下，化学发光强度在0.008 - 1微克/毫升范围内与辛弗林浓度成正比。辛弗林的检测限为1.6纳克/毫升（3σ），0.5微克/毫升辛弗林的相对标准偏差（n = 11）为2.6%。该方法应用于草药产品、柑橘类水果和生物流体中辛弗林的测定。回收率令人满意（90 - 102%）。该方法给出的结果与高效液相色谱 - 紫外法给出的结果高度一致。
