金黄色葡萄球菌游离蛋氨酸 -（R）- 亚砜还原酶的结晶及初步X射线晶体学分析
Crystallization and preliminary X-ray crystallographic analysis of free methionine-(R)-sulfoxide reductase from Staphylococcus aureus.
作者信息
Bong Seoung Min, Moon Jin Ho, Kim Hwa Young, Kim Hong Seok, Chi Young Min, Kim Augustine Yonghwi
机构信息
Korea University, Seoul, Republic of Korea.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt 11):1120-2. doi: 10.1107/S1744309109037105. Epub 2009 Oct 30.
Abstract
Free methionine-(R)-sulfoxide reductase (fRMsr) catalyzes the reduction of the free form of methionine-(R)-sulfoxide back to free methionine. The fRMsr protein from Staphylococcus aureus was overexpressed in Escherichia coli, purified and crystallized at 295 K using ammonium sulfate as a precipitant. Diffraction data were collected to 1.7 angstrom resolution from a native crystal using synchrotron radiation. The crystal belonged to the hexagonal space group P6(1)22, with unit-cell parameters a = b = 89.84, c = 88.75 angstrom, alpha = beta = 90, gamma = 120 degrees. Assuming the presence of one molecule in the asymmetric unit, the calculated Matthews coefficient value was 2.21 angstrom(3) Da(-1), with a solvent content of 57.1%.
摘要
游离型蛋氨酸 -（R）- 亚砜还原酶（fRMsr）催化游离形式的蛋氨酸 -（R）- 亚砜还原回游离蛋氨酸。金黄色葡萄球菌的fRMsr蛋白在大肠杆菌中过表达，经纯化后，以硫酸铵作为沉淀剂，于295 K下进行结晶。利用同步辐射从天然晶体收集到分辨率为1.7埃的衍射数据。该晶体属于六方空间群P6(1)22，晶胞参数a = b = 89.84，c = 88.75埃，α = β = 90°，γ = 120°。假设不对称单元中存在一个分子，计算得到的马修斯系数值为2.21埃³Da⁻¹，溶剂含量为57.1%。
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Structural and kinetic analysis of free methionine-R-sulfoxide reductase from Staphylococcus aureus: conformational changes during catalysis and implications for the catalytic and inhibitory mechanisms.金黄色葡萄球菌游离甲硫氨酸-R-亚砜还原酶的结构和动力学分析：催化过程中的构象变化及其对催化和抑制机制的影响。
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本文引用的文献
1
The methionine sulfoxide reductases: Catalysis and substrate specificities.甲硫氨酸亚砜还原酶：催化作用与底物特异性
Arch Biochem Biophys. 2008 Jun 15;474(2):266-73. doi: 10.1016/j.abb.2008.02.007. Epub 2008 Feb 13.
2
Free methionine-(R)-sulfoxide reductase from Escherichia coli reveals a new GAF domain function.来自大肠杆菌的游离甲硫氨酸 -（R）- 亚砜还原酶揭示了一种新的GAF结构域功能。
Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9597-602. doi: 10.1073/pnas.0703774104. Epub 2007 May 29.
3
Structural analysis of a set of proteins resulting from a bacterial genomics project.对一个细菌基因组计划所产生的一组蛋白质进行结构分析。
Proteins. 2005 Sep 1;60(4):787-96. doi: 10.1002/prot.20541.
4
The three-dimensional structures of peptide methionine sulfoxide reductases: current knowledge and open questions.肽甲硫氨酸亚砜还原酶的三维结构：当前认知与未决问题
Biochim Biophys Acta. 2005 Jan 17;1703(2):249-60. doi: 10.1016/j.bbapap.2004.09.008.
5
The enzymology and biochemistry of methionine sulfoxide reductases.甲硫氨酸亚砜还原酶的酶学与生物化学
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6
Methionine sulfoxide reductases: ubiquitous enzymes involved in antioxidant defense, protein regulation, and prevention of aging-associated diseases.蛋氨酸亚砜还原酶：参与抗氧化防御、蛋白质调节及预防衰老相关疾病的普遍存在的酶。
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10
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