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The TetR-type regulator AtsR is involved in multidrug response in Corynebacterium glutamicum.TetR 型调控器 AtsR 参与谷氨酸棒杆菌的多药反应。
Microb Cell Fact. 2022 Jun 21;21(1):123. doi: 10.1186/s12934-022-01850-0.
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Similarities in the structure of the transcriptional repressor AmtR in two different space groups suggest a model for the interaction with GlnK.两种不同空间群中转录阻遏物AmtR的结构相似性提示了其与GlnK相互作用的模型。
Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):146-151. doi: 10.1107/S2053230X17002485. Epub 2017 Feb 21.

本文引用的文献

1
DNA binding by Corynebacterium glutamicum TetR-type transcription regulator AmtR.谷氨酸棒杆菌TetR型转录调节因子AmtR与DNA的结合
BMC Mol Biol. 2009 Jul 23;10:73. doi: 10.1186/1471-2199-10-73.
2
A combination of metabolome and transcriptome analyses reveals new targets of the Corynebacterium glutamicum nitrogen regulator AmtR.代谢组学和转录组学分析相结合揭示了谷氨酸棒杆菌氮调节因子AmtR的新靶点。
J Biotechnol. 2009 Mar 10;140(1-2):68-74. doi: 10.1016/j.jbiotec.2008.10.009. Epub 2008 Nov 12.
3
A short history of SHELX.SHELX简史。
Acta Crystallogr A. 2008 Jan;64(Pt 1):112-22. doi: 10.1107/S0108767307043930. Epub 2007 Dec 21.
4
Nitrogen control in Corynebacterium glutamicum: proteins, mechanisms, signals.谷氨酸棒杆菌中的氮控制：蛋白质、机制、信号
J Microbiol Biotechnol. 2007 Feb;17(2):187-94.
5
Systems biology for industrial strains and fermentation processes--example: amino acids.工业菌株与发酵过程的系统生物学——实例：氨基酸
J Biotechnol. 2007 Apr 30;129(2):181-90. doi: 10.1016/j.jbiotec.2007.01.031. Epub 2007 Feb 11.
6
Nitrogen metabolism and nitrogen control in corynebacteria: variations of a common theme.棒状杆菌中的氮代谢与氮调控：一个共同主题的变体
J Mol Microbiol Biotechnol. 2007;12(1-2):131-8. doi: 10.1159/000096468.
7
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators.乳酸乳球菌Dha操纵子的调控：与Tetr转录调节因子家族遵循的规则存在偏差。
J Biol Chem. 2006 Aug 11;281(32):23129-37. doi: 10.1074/jbc.M603486200. Epub 2006 Jun 7.
8
Regulation of AmtR-controlled gene expression in Corynebacterium glutamicum: mechanism and characterization of the AmtR regulon.谷氨酸棒杆菌中AmtR调控的基因表达调控：AmtR调控子的机制与特性
Mol Microbiol. 2005 Oct;58(2):580-95. doi: 10.1111/j.1365-2958.2005.04855.x.
9
The TetR family of transcriptional repressors.转录抑制因子的TetR家族。
Microbiol Mol Biol Rev. 2005 Jun;69(2):326-56. doi: 10.1128/MMBR.69.2.326-356.2005.
10
Regulation of GlnK activity: modification, membrane sequestration and proteolysis as regulatory principles in the network of nitrogen control in Corynebacterium glutamicum.谷氨酰胺激酶（GlnK）活性的调控：修饰、膜隔离和蛋白水解作为谷氨酸棒杆菌氮控制网络中的调控原则
Mol Microbiol. 2004 Oct;54(1):132-47. doi: 10.1111/j.1365-2958.2004.04247.x.

谷氨酸棒杆菌全局氮调节因子AmtR的结晶及初步晶体学分析

Crystallization and preliminary crystallographic analysis of the global nitrogen regulator AmtR from Corynebacterium glutamicum.

作者信息

Hasselt Kristin, Sevvana Madhumati, Burkovski Andreas, Muller Yves A

机构信息

Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt 11):1123-7. doi: 10.1107/S174430910903663X. Epub 2009 Oct 30.

DOI:10.1107/S174430910903663X
PMID:19923732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2777040/
Abstract

AmtR, a member of the TetR family of transcription regulators, is a global regulator of nitrogen control in Corynebacterium glutamicum. Unlike other TetR-family members, which are regulated by small-molecule effectors, AmtR is regulated by a protein called GlnK. It has been shown that a GlnK trimer has to become adenylylated prior to formation of a complex with AmtR. The physiological function of AmtR has been very well studied, but structural characterization of the mechanistic aspects of AmtR-regulated transcription has yet to be accomplished. AmtR has successfully been crystallized in space group P2(1)2(1)2, with six molecules in the asymmetric unit and unit-cell parameters a = 153.34, b = 163.10, c = 51.93 angstrom . Preliminary phases were obtained using Se-SAD.

摘要

AmtR是转录调节因子TetR家族的成员，是谷氨酸棒杆菌氮控制的全局调节因子。与其他受小分子效应物调节的TetR家族成员不同，AmtR受一种名为GlnK的蛋白质调节。研究表明，GlnK三聚体在与AmtR形成复合物之前必须进行腺苷酸化。AmtR的生理功能已得到很好的研究，但对AmtR调节转录的机制方面的结构表征尚未完成。AmtR已成功在空间群P2(1)2(1)2中结晶，不对称单元中有六个分子，晶胞参数a = 153.34，b = 163.10，c = 51.93埃。使用硒单波长反常散射法获得了初步相位。