Department of General Surgery, University of Heidelberg, Heidelberg, Germany.
Pancreas. 2010 May;39(4):502-9. doi: 10.1097/MPA.0b013e3181bd6470.
Systemic complications in alcoholic pancreatitis are supposed to be aggravated by inflammatory liver damage. Resident macrophages including hepatic Kupffer cells play a pivotal role in mediating systemic complications in severe necrotizing pancreatitis (SNP). The aim of this study was to evaluate the effects of Kupffer cell inhibition on the inflammatory liver damage in experimental alcoholic pancreatitis.
Rats were fed with either alcohol or control diet for 6 weeks before induction of SNP. Animals were allocated into 4 groups: healthy controls, controls with SNP, SNP with gadolinium chloride or glycine (permanent vs temporary inhibition of hepatic Kupffer cells) prophylaxis. Hepatic microcirculation and morphologic damage of the liver and pancreas were assessed.
Alcohol feeding and SNP increased hepatic and pancreatic injury compared with SNP alone. Gadolinium chloride and glycine improved hepatic microcirculation. In contrast, pancreatic and hepatic morphological damage was reduced by gadolinium chloride but not by glycine.
Alcohol exposure aggravates hepatic and pancreatic injury in SNP. Gadolinium chloride reduces both microcirculatory and morphological damage, whereas glycine did not improve histological damage.
酒精性胰腺炎的全身并发症据推测会因炎症性肝损伤而加重。包括肝库普弗细胞在内的固有巨噬细胞在介导严重坏死性胰腺炎（SNP）的全身并发症中起关键作用。本研究旨在评估库普弗细胞抑制对实验性酒精性胰腺炎炎症性肝损伤的影响。
在诱导 SNP 之前，大鼠先接受酒精或对照饮食 6 周。动物分为 4 组：健康对照组、对照 SNP 组、SNP 加钆氯化物或甘氨酸（肝库普弗细胞永久或暂时抑制）预防组。评估肝脏和胰腺的肝微循环和形态损伤。
与 SNP 相比，酒精喂养和 SNP 增加了肝和胰腺损伤。钆氯化物和甘氨酸改善了肝微循环。相比之下，钆氯化物减少了胰腺和肝的形态损伤，但甘氨酸没有。
酒精暴露加重 SNP 中的肝和胰腺损伤。钆氯化物可减少微循环和形态损伤，而甘氨酸不能改善组织学损伤。
