Chongqing Key Laboratory of Hepatobiliary Surgery and Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing Medical University, 74 Linjiang Road, Chongqing 400010, China.
Inflammation. 2012 Apr;35(2):690-701. doi: 10.1007/s10753-011-9362-0.
This study was undertaken to clarify the effects of taurine on liver injury in rats with severe acute pancreatitis (SAP). Rats were randomly assigned to three groups: a sham operation (SO), a SAP (established by infusion of 5% taurocholate), and a SAP given taurine (Taur). At 12 and 24 h post-operation, taurine pretreatment significantly attenuated hepatic tissue injury induced by SAP, and concurrently, serum alanine aminotransferase, aspartate transaminase, and amylase levels were significantly reduced by taurine pretreatment. Compared with the SO group, the total and phosphorylated p38 mitogen-activated protein kinase (p38 MAPK) expression and nuclear factor-κB (NF-κB) activity of Kupffer cells (KCs) were significantly higher in the SAP group, but taurine pretreatment inhibited the total and phosphorylated p38 MAPK expression and NF-κB activity of KCs in the SAP group. The increase of tumor necrosis factor-α and interleukin-lβ in cultured supernate of the SAP rat-derived KCs was also significantly inhibited by taurine pretreatment. These results suggest that taurine pretreatment ameliorated liver injury in rats with SAP mainly by inhibiting phosphorylated p38 MAPK and NF-κB activity in KCs, which may play an important role in liver injury.
本研究旨在阐明牛磺酸对重症急性胰腺炎（SAP）大鼠肝损伤的影响。大鼠被随机分为三组：假手术（SO）组、SAP 组（通过输注 5%牛磺胆酸钠建立）和 SAP 加牛磺酸（Taur）组。术后 12 和 24 小时，牛磺酸预处理显著减轻了 SAP 诱导的肝组织损伤，同时，牛磺酸预处理显著降低了血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶和淀粉酶水平。与 SO 组相比，SAP 组的总及磷酸化 p38 丝裂原活化蛋白激酶（p38 MAPK）表达和核因子-κB（NF-κB）活性以及库普弗细胞（KCs）的核因子-κB（NF-κB）活性明显升高，但牛磺酸预处理抑制了 SAP 组中总及磷酸化 p38 MAPK 表达和 NF-κB 活性。牛磺酸预处理还显著抑制了 SAP 大鼠来源的 KCs 培养上清液中肿瘤坏死因子-α和白细胞介素-1β的增加。这些结果表明，牛磺酸预处理主要通过抑制 KCs 中磷酸化 p38 MAPK 和 NF-κB 活性来改善 SAP 大鼠的肝损伤，这可能在肝损伤中起重要作用。
