UNESP-Univ Estadual Paulista, Instituto de Química, Departamento de Química Orgânica, Araraquara, São Paulo, Brazil.
Molecules. 2009 Nov 16;14(11):4644-54. doi: 10.3390/molecules14114644.
Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-beta-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.
采用高效液相色谱法结合 DAD 检测对样品进行定量分析，开发出一种方法，用于分析从不同属的甲醇提取物中分离出的芦丁、6-甲氧基芹菜素和 6-甲氧基芹菜素-7-O-β-D-吡喃葡萄糖苷：葱属、Leiothix 和水玉簪属（水玉簪科）。评估了该方法的线性、准确性和日间精密度。校准曲线呈线性。分析样品中黄酮类化合物的回收率为 96.3%至 98.5%。样品分析中黄酮类化合物定量分析的变异系数百分比低于 5%。对这 5 种水玉簪属植物甲醇提取物的抗菌活性进行了测定，以评估其对金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、肠炎沙门氏菌、酿酒酵母和白色念珠菌的抑制作用。测定的 MIC 值范围为 1.25 至 10.00mg/mL。黄酮类化合物的含量表明，水玉簪属植物可能是生产天然植物药的有前途的化合物来源。
