一种新型5'-碘核苷可实现单链和双链DNA的高效非酶连接。
A Novel 5'-Iodonucleoside Allows Efficient Nonenzymatic Ligation of Single-stranded and Duplex DNAs.
作者信息
Xu Yanzheng, Kool Eric T
机构信息
Department of Chemistry, University of Rochester, Rochester, NY 14627.
出版信息
Tetrahedron Lett. 1997 Aug 11;38(32):5595-5598. doi: 10.1016/S0040-4039(97)01266-5.
Abstract
A new iodothymidine phosphoramidite enables the placement of a 5'-iodide into oligonucleotides; the iodide is stable to ammonia deprotection and allows nonenzymatic ligations of DNA.
摘要
一种新型碘胸苷亚磷酰胺可将5'-碘化物引入寡核苷酸中；该碘化物对氨解保护稳定，并能实现DNA的非酶连接。
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