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一种新型5'-碘核苷可实现单链和双链DNA的高效非酶连接。

A Novel 5'-Iodonucleoside Allows Efficient Nonenzymatic Ligation of Single-stranded and Duplex DNAs.

作者信息

Xu Yanzheng, Kool Eric T

机构信息

Department of Chemistry, University of Rochester, Rochester, NY 14627.

出版信息

Tetrahedron Lett. 1997 Aug 11;38(32):5595-5598. doi: 10.1016/S0040-4039(97)01266-5.

DOI:10.1016/S0040-4039(97)01266-5
PMID:19924262
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2778307/
Abstract

A new iodothymidine phosphoramidite enables the placement of a 5'-iodide into oligonucleotides; the iodide is stable to ammonia deprotection and allows nonenzymatic ligations of DNA.

摘要

一种新型碘胸苷亚磷酰胺可将5'-碘化物引入寡核苷酸中；该碘化物对氨解保护稳定，并能实现DNA的非酶连接。

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A Novel 5'-Iodonucleoside Allows Efficient Nonenzymatic Ligation of Single-stranded and Duplex DNAs.一种新型5'-碘核苷可实现单链和双链DNA的高效非酶连接。
Tetrahedron Lett. 1997 Aug 11;38(32):5595-5598. doi: 10.1016/S0040-4039(97)01266-5.
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The chemical end-ligation of homopyrimidine oligodeoxyribonucleotides within a DNA triple helix.DNA三螺旋内同嘧啶寡脱氧核糖核苷酸的化学末端连接
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Halo sugar nucleosides. I. Iodination of the primary hydroxyl groups of nucleosides with methyltriphenoxyphosphonium iodide.卤代糖核苷。I. 用甲基三苯氧基碘化鏻对核苷的伯羟基进行碘化反应。
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