Department of Bioscience and Nutrition, Karolinska Institutet, Stockholm, Sweden.
PLoS One. 2009 Nov 16;4(11):e7844. doi: 10.1371/journal.pone.0007844.
Preimplantation development is a crucial step in early human development. However, the molecular basis of human preimplantation development is not well known.
By applying microarray on 397 human oocytes and embryos at six developmental stages, we studied the transcription dynamics during human preimplantation development.
We found that the preimplantation development consisted of two main transitions: from metaphase-II oocyte to 4-cell embryo where mainly the maternal genes were expressed, and from 8-cell embryo to blastocyst with down-regulation of the maternal genes and up-regulation of embryonic genes. Human preimplantation development proved relatively autonomous. Genes predominantly expressed in oocytes and embryos are well conserved during evolution.
Our database and findings provide fundamental resources for understanding
植入前发育是人类早期发育的关键步骤。然而，人类植入前发育的分子基础尚不清楚。
通过在六个发育阶段的 397 个人卵和胚胎上应用微阵列，我们研究了人类植入前发育过程中的转录动态。
我们发现植入前发育由两个主要的转变组成：从减数分裂 II 期卵母细胞到 4 细胞胚胎，主要表达母系基因，从 8 细胞胚胎到囊胚，母系基因下调，胚胎基因上调。人类植入前发育表现出相对自主性。在进化过程中，在卵母细胞和胚胎中主要表达的基因得到了很好的保守。
我们的数据库和发现为理解提供了基础资源。
