Department of Microbiology, Oregon State University, 139 Oak Creek Building, 97331 Corvallis, OR, USA.
Curr Microbiol. 2010 Mar;60(3):229-35. doi: 10.1007/s00284-009-9530-8.
Tall fescue toxicosis and ergot alkaloids cost U.S. livestock producers approximately one billion dollars in annual livestock production loss annually. Ergovaline (EV) is the tall fescue alkaloid primarily responsible for clinical disease in livestock. Since native ruminal microorganisms have not been attributed to the detoxification of EV, finding detoxifying microbes from other environments is desirable. One possible source for potential microorganisms that can degrade EV is the anaerobic gut of the earthworm, Eisenia fetida. This study describes a comparative microbial analysis of earthworm digestive tracts receiving 10,000 ppb EV (E+ treatment) when compared with a control treatment with no detectable amounts of EV (E- treatment). An HPLC assay determined a 25% loss of EV from the E+ treatment was microbial in nature. A community microbiomic approach of constructing 16S-rRNA gene clone libraries was used to compare the microbes affected by the two treatments. RDPII tools such as Classifier and Libcompare were used in the analysis of 16S sequences. DOTUR analysis was used to examine the richness and diversity of the two microbial populations in these experiments. The results indicate there are few significant differences in the microbial community structure between the two microbiomes.
黑麦草中毒和麦角生物碱每年使美国牲畜生产者在牲畜生产损失上花费约 10 亿美元。麦角碱（EV）是主要导致牲畜临床疾病的黑麦草生物碱。由于天然瘤胃微生物尚未被归因于 EV 的解毒，因此从其他环境中寻找解毒微生物是可取的。一种可能的潜在微生物来源是蚯蚓的厌氧肠道，即赤子爱胜蚓。本研究描述了对接收 10000ppbEV（E+处理）的蚯蚓消化道进行微生物比较分析，与未检测到 EV 的对照处理（E-处理）进行比较。HPLC 测定表明，E+处理中 EV 的 25%损失具有微生物性质。构建 16S-rRNA 基因克隆文库的群落微生物组学方法用于比较两种处理方式下受影响的微生物。使用 RDPII 工具（如 Classifier 和 Libcompare）对 16S 序列进行分析。DOTUR 分析用于检查这两个实验中两种微生物种群的丰富度和多样性。结果表明，两种微生物组之间的微生物群落结构几乎没有显著差异。
