National Institute for Nanotechnology, National Research Council Canada, Edmonton, Alberta, Canada.
Electrophoresis. 2009 Dec;30(24):4237-44. doi: 10.1002/elps.200900334.
Microchip-based bead-packed columns for electrochromatography are described for several types of stationary phases. Chromatography columns 2 mm in length were used for the separation of proteins and peptides by size- and ion-exchange modes of separation, respectively. In size-exclusion electrochromatograpgy, FITC-IgG and FITC-insulin were baseline resolved in less than 10 s, with efficiencies of up to 139,000 plates/m for FITC-insulin. In strong cation-exchange electrochromatography, a mixture of three fluorescently labeled peptides was baseline resolved in less than 40 s, with efficiencies up to 400,000 plates/m. The RSD for the analytes retention times were<3% in both size-exclusion and ion-exchange modes of separations. The use of a 1-mm-long reverse-phase column for the semiquantitative evaluation of pharmaceutical formulations in drug solubility tests illustrates the use of this microfluidic chip-based electrochromatographic approach to drug development.
基于微芯片的填充珠电色谱柱用于多种类型的固定相。分别采用尺寸排阻和离子交换模式分离的 2mm 长色谱柱用于蛋白质和肽的分离。在尺寸排阻电色谱中，FITC-IgG 和 FITC-胰岛素在不到 10s 内实现基线分离，FITC-胰岛素的理论塔板数高达 139,000 块/m。在强阳离子交换电色谱中，三种荧光标记肽的混合物在不到 40s 内实现基线分离，理论塔板数高达 400,000 块/m。在尺寸排阻和离子交换模式分离中，分析物保留时间的 RSD 均<3%。使用 1mm 长的反相柱对半定量评估药物溶解度试验中的药物制剂说明了这种基于微流控芯片的电色谱方法在药物开发中的应用。
