Wang Yucong, Zhang Zhichao, Zhang Lei, Li Feng, Chen Lei, Wan Qian-Hong
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Anal Chem. 2007 Jul 1;79(13):5082-6. doi: 10.1021/ac070288b. Epub 2007 Jun 1.
Fritless packed beds comprised of magnetically responsive octadecylsilane bonded silica particles have been constructed for reversed-phase electrochromatography. The magnetic particles were immobilized in the capillary by applying an external magnetic field transverse to the direction of electroosmotic flow. Being subjected to the interplay of fluid dragging and magnetic forces, the initial loosely packed particle assembly was compacted into a uniform packing structure. The magnetically immobilized beds obtained were used as stationary phases for separation of neutral compounds, with retention behavior and column efficiency similar to those of slurry-packed columns. The results suggest that the magnetic attraction approach to fritless column packing may be used for construction of advanced chip-based chromatography, especially in complex architectures comprising curved and intersecting channels.
由磁性响应十八烷基硅烷键合硅胶颗粒组成的无烧结填充床已被构建用于反相电色谱。通过施加与电渗流方向垂直的外部磁场，磁性颗粒被固定在毛细管中。在流体拖拽力和磁力的共同作用下，最初松散堆积的颗粒组件被压实成均匀的填充结构。所获得的磁性固定床用作分离中性化合物的固定相，其保留行为和柱效率与淤浆填充柱相似。结果表明，用于无烧结柱填充的磁吸引方法可用于构建先进的基于芯片的色谱，特别是在包含弯曲和交叉通道的复杂结构中。
