Lesueur L, Edery M, Ali S, Paly J, Kelly P A, Djiane J
Unité d'Endocrinologie Moleculaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):824-8. doi: 10.1073/pnas.88.3.824.
The biological activities of long and short forms of the prolactin receptor have been compared. These two receptors expressed in mammalian cells were shown to bind prolactin with equal high affinity. The ability of these different forms to transduce the hormonal message was estimated by their capacity to stimulate transcription by using the promoter of a milk protein gene fused to the chloramphenicol acetyltransferase (CAT) coding sequence. Experiments were performed in serum-free conditions to avoid the effect of lactogenic factors present in serum. An approximately 17-fold induction of CAT activity was obtained in the presence of prolactin when the long form of the prolactin receptor was expressed, whereas no induction was observed when the short form was expressed. The present results clearly establish that only the long form of the prolactin receptor is involved in milk protein gene transcription.
对催乳素受体长、短两种形式的生物学活性进行了比较。在哺乳动物细胞中表达的这两种受体显示出与催乳素结合时具有同等的高亲和力。通过利用与氯霉素乙酰转移酶（CAT）编码序列融合的乳蛋白基因启动子来刺激转录的能力，评估了这些不同形式的受体转导激素信号的能力。实验在无血清条件下进行，以避免血清中存在的催乳因素的影响。当表达催乳素受体的长形式时，在催乳素存在的情况下，CAT活性获得了约17倍的诱导，而当表达短形式时未观察到诱导现象。目前的结果清楚地表明，只有催乳素受体的长形式参与乳蛋白基因的转录。
