Lesueur L, Edery M, Paly J, Clark J, Kelly P A, Djiane J
Unité d'Endocrinologie Moléculaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Mol Cell Endocrinol. 1990 May 28;71(1):R7-12. doi: 10.1016/0303-7207(90)90079-n.
A functional biological system was developed by cotransfecting mammalian cell lines with the cDNA of the prolactin receptor (PRL-R) and a fusion gene containing the promoter of the milk protein, ovine beta-lactoglobulin linked to the coding sequence of the chloramphenicol acetyltransferase (CAT) gene. Surprisingly, this system is effective even if a non-mammary cell line is used, since Chinese hamster ovary (CHO) cells transfected both transiently and stably with PRL-R cDNA respond to PRL, as observed by stimulation of the reporter gene. This newly developed system should help precisely define the functional domains of both the PRL-R molecule and of the regulatory elements of a PRL target gene.
通过将催乳素受体（PRL-R）的cDNA与一个融合基因共转染哺乳动物细胞系构建了一个功能性生物系统，该融合基因包含与氯霉素乙酰转移酶（CAT）基因编码序列相连的乳蛋白（绵羊β-乳球蛋白）启动子。令人惊讶的是，即使使用非乳腺细胞系，该系统也是有效的，因为用PRL-R cDNA瞬时和稳定转染的中国仓鼠卵巢（CHO）细胞对PRL有反应，这可通过报告基因的激活来观察到。这个新开发的系统应该有助于精确界定PRL-R分子的功能结构域以及PRL靶基因调控元件的功能结构域。
