Institute of Materials Science & Technology (IMT), Friedrich-Schiller-University (FSU), Löbdergraben 32, 07743 Jena, Germany.
Small. 2010 Feb 5;6(3):465-70. doi: 10.1002/smll.200900670.
A novel approach is developed to address the requirement of multiple stamps and inks for microcontact printing (microCP) onto different substrate surfaces. This approach relies on microCP one divalent molecule, which is able to form Janus self-assembled monolayers (JSAMs) with a labile cleavable centre, thus providing a facile method for the chemical derivatization of different substrate surfaces. This study presents an answer to the challenges presented within a highly versatile application, microCP. N-(3-diethylphosphatoxy)propyl-11-mercaptoundecanamide is used for the first time as an ink for microCP onto both gold and titanium oxide surfaces, utilizing the same polydimethylsiloxane stamp. Following printing, the JSAMs are enzymatically treated on these two different substrates to reveal different functional groups. The newly formed surfaces are subjected to additional surface reactions and used for the chemisorption of bovine serum albumin. At each stage, these JSAMs are characterized by X-ray photoelectron spectroscopy and dynamic water-contact-angle measurements. Confocal laser scanning microscopy is used for the characterization of the adsorbed proteins.
开发了一种新方法来满足微接触印刷（microCP）到不同基底表面所需的多个印章和油墨的要求。这种方法依赖于 microCP 一种二价分子，它能够与不稳定的可裂解中心形成 Janus 自组装单层（JSAMs），从而为不同基底表面的化学衍生化提供了一种简便的方法。本研究为高度通用的应用微 CP 中提出的挑战提供了答案。首次将 N-(3-二乙膦氧基)丙基-11-巯基十一烷酰胺用作微 CP 到金和氧化钛表面的油墨，利用相同的聚二甲基硅氧烷印章。印刷后，在这两种不同的基底上通过酶处理来揭示不同的官能团。新形成的表面进行额外的表面反应，并用于牛血清白蛋白的化学吸附。在每个阶段，这些 JSAMs 都通过 X 射线光电子能谱和动态水接触角测量进行表征。共焦激光扫描显微镜用于吸附蛋白质的表征。
