European School of Molecular Medicine (SEMM), IFOM-IEO Campus, Via Adamello 16, 20139, Milan, Italy.
Biofabrication. 2012 Jun;4(2):025001. doi: 10.1088/1758-5082/4/2/025001. Epub 2012 Mar 12.
The contamination of implant devices as a result of biofilm formation through bacterial infection has instigated major research in this area, particularly to understand the mechanism of bacterial cell/implant surface interactions and their preventions. In this paper, we demonstrate a controlled method of nanostructured titanium oxide surface synthesis using supersonic cluster beam depositions. The nanoscale surface characterization using atomic force microscopy and a profilometer display a regulated evolution in nanomorphology and physical properties. X-ray photoelectron spectroscopy analyses display a stoichiometric nanostructured TiO(2) film. Measurement of the water contact angle shows a nominal increase in the hydrophilic nature of ns-TiO(2) films, whereas the surface energy increases with decreasing contact angle. Bacterial species Staphylococcus aureus and Escherichia coli interaction with nanostructured surfaces shows an increase in adhesion and biofilm formation with increasing nanoscale morphological properties. Conversely, limiting ns-TiO(2) film distribution to micro/nanopatterned designed substrates integrated with bovine serum albumin functionalization leads to a reduction in biofilm formations due to a globally decreased bacterial cell-surface interaction area. The results have potential implications in inhibiting bacterial colonization and promoting mammalian cell-implant interactions.
由于细菌感染导致生物膜形成而对植入设备造成的污染，促使该领域进行了大量研究，特别是为了了解细菌细胞/植入物表面相互作用及其预防的机制。在本文中，我们展示了一种使用超声分子束沉积来控制合成纳米结构氧化钛表面的方法。原子力显微镜和轮廓仪对纳米级表面特性的分析显示出纳米形貌和物理性质的规则演变。X 射线光电子能谱分析显示出具有化学计量比的纳米结构 TiO(2) 薄膜。水接触角的测量表明 ns-TiO(2) 薄膜的亲水性名义上有所增加，而表面能随接触角的减小而增加。与纳米结构表面相互作用的金黄色葡萄球菌和大肠杆菌等细菌物种的粘附和生物膜形成随着纳米形貌特性的增加而增加。相反，由于细菌细胞-表面相互作用面积的全局减少，将 ns-TiO(2) 薄膜分布限制在与牛血清白蛋白功能化集成的微/纳米图案化设计基板上，会导致生物膜形成减少。这些结果可能对抑制细菌定植和促进哺乳动物细胞-植入物相互作用具有重要意义。
