Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, 1201 Welch Road, Stanford, California 94305-5484, USA.
Angew Chem Int Ed Engl. 2009;48(51):9658-62. doi: 10.1002/anie.200903627.
This communication describes a novel protein labeling method that uses a single amino-acid tag — N-terminal cysteine residue — and small-molecule probes carrying the cyanobenzothiazole unit for specific labeling of proteins in vitro and at the surface of live cells. This simple ligation reaction proceeds with a high degree of specificity in physiological conditions, and should offer an important alternative to currently available protein labeling methods.
本通讯描述了一种新颖的蛋白质标记方法，该方法使用单个氨基酸标签——N 端半胱氨酸残基——和携带氰基苯并噻唑单元的小分子探针，用于体外和活细胞表面的蛋白质的特异性标记。这种简单的连接反应在生理条件下具有高度特异性，应该为现有的蛋白质标记方法提供一个重要的替代方案。
