Dept. of Biotechnology, Delft University of Technology and Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628 BC, Delft, The Netherlands.
Biotechnol Prog. 2010 Jan-Feb;26(1):293-300. doi: 10.1002/btpr.315.
Aerobic batch cultivations of Candida utilis were carried out in two micro bioreactors with a working volume of 100 muL operated in parallel. The dimensions of the micro bioreactors were similar as the wells in a 96-well microtiter plate, to preserve compatibility with the current high-throughput cultivation systems. Each micro bioreactor was equipped with an electrochemical sensor array for the online measurement of temperature, pH, dissolved oxygen, and viable biomass concentration. Furthermore, the CO(2) production rate was obtained from the online measurement of cumulative CO(2) production during the cultivation. The online data obtained by the sensor array and the CO(2) production measurements appeared to be very reproducible for all batch cultivations performed and were highly comparable to measurement results obtained during a similar aerobic batch cultivation carried out in a conventional 4L bench-scale bioreactor. Although the sensor chip certainly needs further improvement on some points, this work clearly shows the applicability of electrochemical sensor arrays for the monitoring of parallel micro-scale fermentations, e.g. using the 96-well microtiterplate format.
在两个 100 μL 工作体积的微生物反应器中进行了产朊假丝酵母的好氧批量培养，这两个微生物反应器平行运行。微生物反应器的尺寸与 96 孔微量滴定板的孔相似，以保持与当前高通量培养系统的兼容性。每个微生物反应器都配备了电化学传感器阵列，用于在线测量温度、pH 值、溶解氧和活生物质浓度。此外，通过在线测量培养过程中累积的 CO2 产量，可以获得 CO2 生成速率。对于所有进行的批量培养，传感器阵列获得的在线数据和 CO2 生成测量结果似乎非常可重复，并且与在类似的在传统的 4L 台式生物反应器中进行的好氧批量培养中获得的测量结果高度可比。尽管传感器芯片在某些方面肯定需要进一步改进，但这项工作清楚地表明电化学传感器阵列适用于监测平行的微尺度发酵，例如使用 96 孔微量滴定板格式。
