Department of Urology, Faculty of Medicine, Mustafa Kemal University, Hatay, Turkey.
Ren Fail. 2009;31(9):843-7. doi: 10.3109/08860220903137517.
Cyclosporine A, an immunosuppressive agent, is widely used after organ transplantation such as the liver and kidney. However, its widespread use is restricted because it has serious toxic effects on the kidney. Caffeic acid phenethyl ester (CAPE) is a natural product with potent anti-inflammatory, antitumor, and antioxidant activities, and it attenuates inflammation and lipid peroxidation induced by ischemia-reperfusion injury. The purpose of the present study was to investigate the effects of CAPE on cyclosporine A (CsA)-induced nephrotoxicity.
Rats were divided into four groups and treated with saline, CAPE, CsA, and CsA + CAPE. Control rats were given saline; the CAPE group was given CAPE (10 micromol/kg/day) for 11 days intraperitoneally; the CsA group was given CsA (15 mg/kg/day) for 10 days subcutaneously; and the CsA+CAPE group was given CAPE for 11 days, and rats were s.c. injected with CsA in 0.5 ml of saline once a day for 10 days at the same time.
The administration of CsA alone resulted in higher myeloperoxidase (MPO) activity, lipid peroxidation, superoxide dismutase (SOD), and catalase (CAT) than in the control. The enzyme activities except CAT in rats treated with CAPE alone were not changed. CAPE treatment prevented the increase in malondialdehyde (MDA) and increased CAT activity more, but did not affect the activities of MPO and SOD enzymes.
CsA causes renal injury and CAPE prevents CAT- and lipid peroxidation-mediated nephrotoxicity via inhibition of oxidative process.
环孢素 A 是一种免疫抑制剂，广泛用于肝、肾等器官移植后。然而，由于其对肾脏有严重的毒性作用，其广泛应用受到限制。咖啡酸苯乙酯（CAPE）是一种具有强大抗炎、抗肿瘤和抗氧化活性的天然产物，可减轻缺血再灌注损伤引起的炎症和脂质过氧化。本研究旨在探讨 CAPE 对环孢素 A（CsA）诱导的肾毒性的影响。
大鼠分为四组，分别给予生理盐水、CAPE、CsA 和 CsA+CAPE。对照组给予生理盐水；CAPE 组腹腔内给予 CAPE（10 微摩尔/千克/天）11 天；CsA 组皮下给予 CsA（15 毫克/千克/天）10 天；CsA+CAPE 组连续 11 天腹腔内给予 CAPE，同时每天在 0.5 毫升生理盐水中皮下注射 CsA 10 天。
单独给予 CsA 可导致髓过氧化物酶（MPO）活性、脂质过氧化、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）升高，高于对照组。单独给予 CAPE 的大鼠的酶活性除 CAT 外均无变化。CAPE 治疗可预防 MDA 的增加，并增加 CAT 活性，但不影响 MPO 和 SOD 酶的活性。
CsA 可引起肾脏损伤，CAPE 通过抑制氧化过程预防 CAT 和脂质过氧化介导的肾毒性。
