College of Food Science and Nutritional Engineering, China Agricultural University, P. O. Box 40, No.17 Qing hua dong lu, Hai dian, Beijing 100083, PR China.
Int J Food Microbiol. 2010 Jan 31;137(1):67-75. doi: 10.1016/j.ijfoodmicro.2009.10.032. Epub 2009 Nov 6.
Neutralized electrolyzed oxidizing water (NEW) and acidic electrolyzed oxidizing water (AcEW) are electrolyzed oxidizing waters (EOW) that have significantly different fungicidal efficiencies against Aspergillus flavus (A. flavus) (The actuation durations of no survival population to NEW and AcEW were 90s and 120s, respectively.), even when used at the same available chlorine concentration (30ppm). It has been verified by our previous research. This study hypothesized that this difference did not originate from the structure of water but based on the OH radical (OH). It was proved by the UV spectroscopy, (17)O-NMR spectroscopy and electron spin resonance analysis. NEW contains more OH compared with AcEW in the same available chlorine concentration level. The OH that exists in NEW and AcEW was found to have an important fungicidal factor that destroys the cellular structures of the A. flavus conidia. It also damages the cellular normal function of A. flavus conidia that brought about K+ and Mg2+ leakages. The levels of OH that exist in NEW and AcEW could be the important reason that leads to significant fungicidal efficiencies against A. flavus.
中性氧化电位水(NEW)和酸性氧化电位水(AcEW)是两种具有显著不同杀菌效率的氧化电位水(EOW),对黄曲霉(A.flavus)(NEW 和 AcEW 对无存活种群的作用时间分别为 90s 和 120s),即使在相同的有效氯浓度(30ppm)下使用也是如此。这是我们之前的研究已经验证的。本研究假设这种差异不是源自水的结构,而是基于羟基自由基(OH)。通过紫外光谱、(17)O-NMR 光谱和电子自旋共振分析得到了证实。在相同的有效氯浓度水平下,NEW 中所含的 OH 比 AcEW 多。在 NEW 和 AcEW 中存在的 OH 被发现是一种重要的杀菌因子,它破坏了黄曲霉孢子的细胞结构。它还破坏了黄曲霉孢子的细胞正常功能,导致 K+和 Mg2+泄漏。NEW 和 AcEW 中存在的 OH 水平可能是导致对黄曲霉具有显著杀菌效率的重要原因。