电解氧化水对白色念珠菌的杀菌效果及其生化机制。

Fungicidal efficiency of electrolyzed oxidizing water on Candida albicans and its biochemical mechanism.

机构信息

School of Life Sciences and Technology, Tongji University, Shanghai, China.

出版信息

J Biosci Bioeng. 2011 Jul;112(1):86-91. doi: 10.1016/j.jbiosc.2011.03.003. Epub 2011 Apr 1.

DOI:10.1016/j.jbiosc.2011.03.003

Abstract

The fungicidal influencing factors of electrolyzed oxidizing water (EOW) on Candida albicans were investigated by suspension quantitative germicidal tests. Results showed that EOW possessed predominant fungicidal rate on C. albican, as high as consumately 100% after 0.5min duration of 65.5mg/L active available chlorine concentration (ACC). The fungicidal effect was promoted proportionally along with ACC but was inhibited by organic interferential bovine serum albumin (BSA). The fungicidal mechanism was also investigated at a biological molecular level by detecting series of biochemical indices. Fluorescent microscopy showed that almost all C. albicans cells were stained red in 1min, suggesting that cell membrane was one of EOW's action targets. Transmission electron microscopy (TEM) showed that EOW destroyed the cellular protective barriers and imposed some damage upon the nucleus area, which verified EOW's effects on microbial ultra-structures. EOW improved membrane permeabilities with the result that the leakages of cellular inclusions (K(+), proteins and DNA) and the conductivity increased rapidly. The dehydrogenase relative activities of C. albicans decreased by 44.0% after 10min, indicating that EOW also had a destructive effect on cellular dehydrogenase.

摘要

采用悬液定量杀菌试验研究了酸性氧化电位水（EOW）对白色念珠菌的杀菌影响因素。结果表明，EOW 对白色念珠菌具有很高的杀菌率，在 65.5mg/L 有效氯浓度（ACC）、0.5min 作用时间下，杀菌率高达 100%。杀菌效果随 ACC 浓度的增加而增加，但被有机干扰物牛血清白蛋白（BSA）抑制。还通过检测一系列生化指标从生物分子水平上研究了杀菌机制。荧光显微镜观察发现，在 1min 内几乎所有的白色念珠菌细胞都被染成红色，提示细胞膜是 EOW 的作用靶点之一。透射电子显微镜（TEM）显示，EOW 破坏了细胞的保护屏障，并对核区造成了一定的损伤，这验证了 EOW 对微生物超微结构的影响。EOW 提高了细胞膜的通透性，导致细胞内含物（K+、蛋白质和 DNA）的渗漏和电导率迅速增加。10min 后，白色念珠菌的脱氢酶相对活性下降了 44.0%，表明 EOW 对细胞脱氢酶也有破坏作用。