Department of Pharmacology, Gansu College of Traditional Chinese Medicine, No. 35, Dingxi East Road, Lanzhou 730000, PR China.
Biomed Pharmacother. 2010 Oct;64(8):521-6. doi: 10.1016/j.biopha.2009.09.002. Epub 2009 Oct 28.
Polymer nanoparticles have become attractive for its prominent property recent years. In this paper, antitumor effects of N-succinyl-chitosan nanoparticles (NSCNP), a nanoparticles derivated from chitosan, were evaluated in K562 cells, including cell viability comparison, cell morphology analysis, DNA fragmentation detection, cell surface potential and mitochondrial membrane potential (MMP) measurement, intracellular ROS and Ca(2+) concentration evaluation. Our results revealed NSCNP could inhibit the proliferation of K562 with an IC(50) of 14.26 μg/ml (24h); decrease the zeta potential; disrupt the mitochondrial membrane potential; increase ROS generation and Ca(2+) concentration. Cytomorphology study and DNA fragment analysis reveal characteristics of apoptosis and necrosis, indicating that the antitumor effect of NSCNP achieved by necrosis and apoptosis induction in K562 cells.
近年来，聚合物纳米粒子因其显著的性能而备受关注。在本文中，我们评估了源自壳聚糖的纳米粒子 N-琥珀酰壳聚糖纳米粒子（NSCNP）对 K562 细胞的抗肿瘤作用，包括细胞活力比较、细胞形态分析、DNA 片段化检测、细胞表面电位和线粒体膜电位（MMP）测量、细胞内 ROS 和 Ca（2+）浓度评估。我们的结果表明，NSCNP 可以抑制 K562 细胞的增殖，IC（50）为 14.26μg/ml（24 小时）；降低 Zeta 电位；破坏线粒体膜电位；增加 ROS 的产生和 Ca（2+）浓度。细胞形态学研究和 DNA 片段分析显示出凋亡和坏死的特征，表明 NSCNP 通过诱导 K562 细胞的坏死和凋亡发挥抗肿瘤作用。
