University of Manchester, UK.
Cancer Epidemiol. 2009 Dec;33(6):463-8. doi: 10.1016/j.canep.2009.10.010. Epub 2009 Nov 18.
The clinical utility of spectroscopic methods for the diagnosis of cervical cancer is limited by significant inter-patient variation in the spectroscopic properties of the cervix. Improved understanding of the contributions of the components of cervical tissue to the observed spectra would therefore be helpful in the development of spectroscopic approaches to the study of cervical disease in vivo.
In this study, we used organotypic epithelial raft culture as an in vitro model system to analyse the fluorescence properties of the surface squamous epithelium specifically. The spectrum of cervical dysplasia was modelled by producing rafts lined by primary human keratinocytes (PHKs) and the HaCaT, SiHa and CaSki human keratinocyte cell lines and fluorescence emission spectra were recorded at a wide range of excitation wavelengths.
Statistically significant differences in spectral shape were identified between the different rafts at excitation wavelengths between 250nm and 310nm. A graded, differential effect of acetic acid on fluorescence intensity was also observed, consistent with the visible effects of acetic acid on clinical examination at colposcopy.
These data suggest that the development of neoplastic changes in the squamous epithelium of the cervix are associated with alterations in its fluorescence properties and that the application of acetic acid has a demonstrable effect on these properties. Identification of these alterations may aid the discrimination of cervical lesions in vivo.
光谱方法在宫颈癌诊断中的临床应用受到宫颈光谱特征在患者间存在显著差异的限制。因此，深入了解宫颈组织成分对观察到的光谱的贡献，将有助于发展光谱方法来研究体内宫颈疾病。
本研究使用器官型上皮筏培养作为体外模型系统，专门分析表面鳞状上皮的荧光特性。通过用原代人角质形成细胞（PHK）和 HaCaT、SiHa 和 CaSki 人角质形成细胞系构建的筏来模拟宫颈发育不良的光谱，并在广泛的激发波长下记录荧光发射光谱。
在 250nm 至 310nm 的激发波长下，不同筏之间的光谱形状存在统计学上的显著差异。还观察到乙酸对荧光强度的分级、差异效应，这与阴道镜检查时乙酸对临床检查的可见效果一致。
这些数据表明，宫颈鳞状上皮的肿瘤性变化与荧光特性的改变有关，并且乙酸的应用对这些特性有明显的影响。识别这些改变可能有助于体内宫颈病变的鉴别。
