Medical Science Lab, Scuola Superiore Sant'Anna, Piazza Martiri della Libertà 33, Pisa, Italy.
Bioelectrochemistry. 2010 Aug;79(1):136-41. doi: 10.1016/j.bioelechem.2009.10.006. Epub 2009 Nov 2.
The use of controlled electric fields to facilitate cell permeabilisation for enhanced cellular uptake of molecules is well established. The main limitation to the application of this technology in clinical practice is the requirements of high voltages which cause significant cell death in the target tissue. This paper presents a new modality for cell electro-permeabilisation based on the use of carbon nanotubes (CNTs) and external static electric fields. An explanation of the results based on the dielectric response of multiwall CNTs (MWCNTs) to these electric fields is proposed. The experimental data obtained indicate that this method of CNT-enhanced electro-permeabilisation provides an effective means of lowering the electric field voltage required for repairable cell electro-permeabilisation to below 50V/cm and with an efficiency exceeding 80%.
利用控制电场促进细胞通透性，以增强分子的细胞摄取，这一方法已得到广泛认可。该技术在临床实践中的主要应用限制是需要高电压，这会导致靶组织中的细胞大量死亡。本文提出了一种基于使用碳纳米管（CNT）和外部静电场的新型细胞电穿孔模式。基于多壁 CNT（MWCNT）对这些电场的介电响应，对结果进行了解释。所获得的实验数据表明，这种 CNT 增强电穿孔的方法为降低可修复细胞电穿孔所需的电场电压提供了有效手段，可将其降至 50V/cm 以下，效率超过 80%。
