State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing, China.
First Affiliated Hospital, Chongqing Medical Science University, Chongqing, China.
Technol Cancer Res Treat. 2019 Jan 1;18:1533033819876918. doi: 10.1177/1533033819876918.
Multi-walled carbon nanotubes with excellent electrical properties and high aspect ratios can reduce the high field strength required to kill cancer cells with nanosecond pulsed electric fields. For the first time, this article systematically and comprehensively evaluates the effects of various parameters of nanosecond pulsed electric fields combined with multi-walled carbon nanotubes on cell viability. The effects of field strength, (2-10 kV/cm); pulse width, τ (100-500 ns); and pulse number, (5-260) on the viability of A375 human skin cancer cells in the presence of multi-walled carbon nanotubes are studied using the Cell Counting Kit 8 assay. Based on a logistic model, the relationship between cell viability and various parameters is obtained using 1-dimensional nonlinear fitting. The results show a sigmoid-type variation in cell viability with field strength, pulse width, or pulse number. Multivariate scaling analysis shows that the relationship between cell viability and the pulse energy density can be described as a sigmoid type. The introduction of multi-walled carbon nanotubes does not affect the above rules but significantly enhances the killing effect of nanosecond pulsed electric fields, which could effectively improve the electrical safety of nanosecond pulsed electric fields for the treatment of tumors.
多壁碳纳米管具有优异的电学性能和高纵横比，可以降低纳秒脉冲电场杀死癌细胞所需的高强度电场。本文首次系统全面地评估了纳秒脉冲电场与多壁碳纳米管结合的各种参数对细胞活力的影响。使用 Cell Counting Kit 8 测定法研究了场强（2-10 kV/cm）、脉冲宽度τ（100-500 ns）和脉冲数（5-260）对存在多壁碳纳米管时 A375 人皮肤癌细胞活力的影响。基于逻辑斯蒂模型，通过一维非线性拟合获得了细胞活力与各种参数之间的关系。结果表明，细胞活力随场强、脉冲宽度或脉冲数呈类正弦型变化。多元标度分析表明，细胞活力与脉冲能量密度之间的关系可以用类正弦型来描述。多壁碳纳米管的引入并不影响上述规律，但显著增强了纳秒脉冲电场的杀伤效果，这可能有效提高纳秒脉冲电场治疗肿瘤的电气安全性。
