The Third Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
J Clin Lab Anal. 2009;23(6):404-7. doi: 10.1002/jcla.20351.
To determine the prevalence and the diversity of extended-spectrum beta-lactamases (ESBLs) among Escherichia coli isolates in Zhengzhou, China.
Clinical isolates were collected and investigated from the first affiliated hospital of Zhengzhou University and its associated health-care facilities in Zhengzhou, China, during the period from January 2006 to June 2008. Antibiograms were performed on Mueller-Hinton agar plates with the disc-diffusion method and MICs were determined by the agar-dilution method. Total DNA was extracted with a Qiagen mini kit and screened by PCR.
Of 94 nonduplicate ESBL-positive isolates, TEM-type was encoded in 74 and 79% of the ESBL isolates. Fifty-six isolates were SHV type ESBLs. CTX-M-1, CTX-M-14, CTX-M-25, and CTX-M-38 types were encoded in 30, 54, 6, and 4, respectively. OXA-1-type beta-lactamases were encoded in six and OXA-20-type was encoded in two isolates.
We describe a complex ESBL epidemiology. The study revealed a high rate of ESBL-producing E. coli isolates. TEM and CTX-M enzymes dominated in ESBL-positive E. coli isolates in Zhengzhou, China.
在中国郑州，确定产超广谱β-内酰胺酶（ESBLs）的大肠杆菌分离株的流行率和多样性。
本研究于 2006 年 1 月至 2008 年 6 月期间，从中国郑州大学第一附属医院及其附属医疗机构采集临床分离株并进行调查。采用纸片扩散法在 Mueller-Hinton 琼脂平板上进行药敏试验，采用琼脂稀释法测定 MIC。使用 Qiagen mini 试剂盒提取总 DNA，并通过 PCR 进行筛选。
在 94 例非重复的 ESBL 阳性分离株中，TEM 型在 74%和 79%的 ESBL 分离株中被编码。56 株为 SHV 型 ESBLs。CTX-M-1、CTX-M-14、CTX-M-25 和 CTX-M-38 型分别在 30、54、6 和 4 株分离株中被编码。6 株分离株编码 OXA-1 型β-内酰胺酶，2 株分离株编码 OXA-20 型β-内酰胺酶。
我们描述了一种复杂的 ESBL 流行情况。该研究揭示了中国郑州产 ESBL 的大肠杆菌分离株的高发生率。TEM 和 CTX-M 酶在郑州产 ESBL 的大肠杆菌分离株中占主导地位。
