Iabadene Hassen, Messai Yamina, Ammari Houria, Ramdani-Bouguessa Nadjia, Lounes Saliha, Bakour Rabah, Arlet Guillaume
Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene, BP 32 El-Alia, Bab-Ezzouar 16111, Alger, Algérie.
J Antimicrob Chemother. 2008 Jul;62(1):133-6. doi: 10.1093/jac/dkn145. Epub 2008 Apr 1.
The aim of this study is to evaluate the prevalence and diversity of extended-spectrum beta-lactamases (ESBLs) in Enterobacter cloacae clinical isolates collected from Algerian hospitals and to verify the association with qnr genes.
MICs were determined by Etest for isolates giving positive double-disc synergy tests, and all isolates were screened by PCR and sequenced, respectively, for bla(TEM), bla(CTX-M), bla(SHV) and bla(VEB) genes and for qnr genes (qnrA, qnrB, qnrS), using specific primers.
The prevalence of ESBLs was 25/141 (17.7%) with 11, 9, 4 and 1 isolates testing positive for genes encoding CTX-M-15, CTX-M-3, SHV-12 and VEB-1, respectively. Two SHV-12 producers and one CTX-M-15 producer expressed QnrS1, one isolate produced CTX-M-15 and QnrB1 and one SHV-12 producer co-expressed QnrS1 and QnrB4. qnrA was not detected in our collection, and qnr alleles were not detected in non-ESBL-producing isolates.
SHV-12, QnrS1, QnrB1 and QnrB4 were reported for the first time in Algeria. This study also described a co-expression of qnrS1 and qnrB4 by an SHV-12 producer isolate.
本研究旨在评估从阿尔及利亚医院收集的阴沟肠杆菌临床分离株中广谱β-内酰胺酶（ESBLs）的流行情况和多样性，并验证其与qnr基因的相关性。
对双纸片协同试验呈阳性的分离株，采用Etest法测定其最低抑菌浓度（MIC），并分别用特异性引物通过PCR和测序对所有分离株的bla(TEM)、bla(CTX-M)、bla(SHV)和bla(VEB)基因以及qnr基因（qnrA、qnrB、qnrS）进行筛查。
ESBLs的流行率为25/141（17.7%），其中分别有11、9、4和1株编码CTX-M-15、CTX-M-3、SHV-12和VEB-1的基因检测呈阳性。两名SHV-12产酶株和一名CTX-M-15产酶株表达QnrS1，一株分离株同时产生CTX-M-15和QnrB1，一名SHV-12产酶株同时表达QnrS1和QnrB4。在我们的样本中未检测到qnrA，在非ESBLs产生株中也未检测到qnr等位基因。
SHV-12、QnrS1、QnrB1和QnrB4在阿尔及利亚首次报道。本研究还描述了一株SHV-12产酶株同时表达qnrS1和qnrB4。
