Woolley I J, Hutchinson P, Reeder J C, Kazura J W, Cortés A
Department of Infectious Diseases, Monash Medical Centre and Monash University, Clayton, Victoria, Australia.
Immunohematology. 2009;25(2):63-6.
The Duffy antigen receptor for chemokines (DARC or Fy glycoprotein) carries antigens that are important in blood transfusion and is the main receptor used by Plasmodium vivax to invade reticulocytes. Southeast Asian ovalocytosis (SAO) results from an alteration in RBC membrane protein band 3 and is thought to mitigate susceptibility to falciparum malaria. Expression of some RBC antigens is suppressed by SAO, and we hypothesized that SAO may also reduce Fy expression, potentiallyleading to reduced susceptibility to vivax malaria. Blood samples were collected from individuals living in the Madang Province of Papua New Guinea. Samples were assayed using a flow cytometry assay for expression of Fy on the surface of RBC and reticulocytes by measuring the attachment of a phycoerythrin-labeled Fy6 antibody. Reticulocytes were detected using thiazole orange. The presence of the SAO mutation was confirmed by PCR. There was a small (approximately 10%) but statistically significant (p=0.049, Mann-Whitney U test) increase in Fy expression on SAO RBC compared with RBC from individuals without this polymorphism: mean Fy expression (mean fluorescence intensity [MFI]) was 10.12 +/- 1.22 for SAO heterozygotes versus an MFI of 8.95 +/- 1.1 for individuals without SAO. For reticulocytes the MFI values were 27.61 +/- 19.12 for SAO heterozygotes and 16.47 +/- 3.81 for controls. SAO is associated with increased and not decreased Fy6 expression so that susceptibility to P. vivax infection is unlikely to be affected.
趋化因子的达菲抗原受体（DARC或Fy糖蛋白）携带在输血中很重要的抗原，并且是间日疟原虫侵入网织红细胞所使用的主要受体。东南亚椭圆形红细胞增多症（SAO）是由红细胞膜蛋白带3的改变引起的，被认为可减轻对恶性疟疾的易感性。SAO会抑制一些红细胞抗原的表达，我们推测SAO也可能降低Fy的表达，从而可能降低对间日疟的易感性。从生活在巴布亚新几内亚马当省的个体中采集血样。通过测量藻红蛋白标记的Fy6抗体的附着情况，使用流式细胞术检测法对红细胞和网织红细胞表面的Fy表达进行检测。使用噻唑橙检测网织红细胞。通过聚合酶链反应（PCR）确认SAO突变的存在。与没有这种多态性的个体的红细胞相比，SAO红细胞上的Fy表达有小幅（约10%）但具有统计学意义（p = 0.049，曼-惠特尼U检验）的增加：SAO杂合子的平均Fy表达（平均荧光强度[MFI]）为10.12±1.22，而没有SAO的个体的MFI为8.95±1.1。对于网织红细胞，SAO杂合子的MFI值为27.61±19.12，对照组为16.47±3.81。SAO与Fy6表达增加而非减少有关，因此对间日疟原虫感染的易感性不太可能受到影响。
