泰国人红细胞趋化因子达菲抗原受体与间日疟原虫感染易感性

RED BLOOD CELL DUFFY ANTIGEN RECEPTOR FOR CHEMOKINES AND SUSCEPTIBILITY TO PLASMODIUM VIVAX INFECTION IN THAIS.

作者信息

Pimpat Yupawadee, Leelawat Kawin, Lek-Uthai Usa

出版信息

Southeast Asian J Trop Med Public Health. 2016 Sep;47(5):885-9.

PMID:29620340

Abstract

Plasmodium vivax is the most prevalent malaria infection in Thailand. P. vivax uses Duffy Antigen Receptor for Chemokines (DARC) or Duffy antigen (Fy) as a receptor for entry into reticulocytes. Polymorphism of DARC exon 2 gene (FYA/FYB) in 40 P. vivax-infected subjects were investigated using nested PCR of blood samples spotted on filter paper collected during August 2013 to November 2013 from various malaria clinics in Thailand. Distribution of DARC genotypes was FYA 62.5%, FYB 20% and FYAB 17.5%, consistent with that of Hardy-Weinberg equation. Mutation G17A was found in both FYA and FYB alleles, resulting in Gyl48 and Asp48 of Fya and Fyb antigen, respectively. Mean parasitemia among the three groups is not statistically different. To the best of our knowledge, this is the first such study in Thailand.

摘要

间日疟原虫是泰国最常见的疟疾感染源。间日疟原虫利用趋化因子的达菲抗原受体（DARC）或达菲抗原（Fy）作为进入网织红细胞的受体。2013年8月至2013年11月期间，从泰国各疟疾诊所收集了滤纸血样，采用巢式PCR对40例间日疟原虫感染患者的DARC外显子2基因（FYA/FYB）多态性进行了研究。DARC基因型分布为FYA 62.5%、FYB 20%和FYAB 17.5%，与哈迪-温伯格方程一致。在FYA和FYB等位基因中均发现了G17A突变，分别导致Fya和Fyb抗原的Gly48和Asp48。三组间的平均疟原虫血症无统计学差异。据我们所知，这是泰国首次进行此类研究。