Angello D A, Headrick J P, Coddington N M, Berne R M
Department of Physiology, University of Virginia Health Sciences Center, Charlottesville 22908.
Am J Physiol. 1991 Jan;260(1 Pt 2):H193-200. doi: 10.1152/ajpheart.1991.260.1.H193.
The effect of adenosine receptor antagonism on function and metabolism was examined in isolated hearts during low flow ischemia and reperfusion. Isovolumic rat hearts perfused at constant flow were subjected to 30 min of ischemia followed by 30 min of reperfusion. Infusion of vehicle or 10 microM 8-phenyltheophylline (8-PT) was initiated 10 min before ischemia and maintained throughout reperfusion. 8-PT infusion had no significant effects on hemodynamic parameters or metabolism preischemia. During ischemia, left ventricular developed pressure declined to approximately 15% of preischemic values in control and 8-PT hearts, and ATP and PCr decreased to approximately 73 and 60% of preischemic values. Inorganic phosphate (Pi) increased to 353 = 41 and 424 +/- 53% of preischemic values in control and 8-PT hearts, respectively. After reperfusion, function recovered to greater than 95% of preischemic levels in control and 8-PT hearts. Unlike control hearts, recovery of metabolites was significantly different during reperfusion in 8-PT hearts (P less than 0.05); ATP, phosphocreatine, and Pi recovered to 82 +/- 8, 71 +/- 8, and 281 +/- 27% of preischemic values, respectively. Venous purine washout was significantly greater (P less than 0.05) during reperfusion in 8-PT hearts (327 +/- 113 nmol) than in control hearts (127 +/- 28 nmol). Blockade of adenosine receptors appears to adversely affect metabolic but not functional recovery in the ischemic-reperfused myocardium.
在低流量缺血和再灌注期间，研究了腺苷受体拮抗剂对离体心脏功能和代谢的影响。以恒定流量灌注的等容大鼠心脏先经历30分钟缺血，然后再灌注30分钟。在缺血前10分钟开始输注溶媒或10微摩尔8-苯基茶碱（8-PT），并在整个再灌注过程中持续输注。输注8-PT对缺血前的血流动力学参数或代谢没有显著影响。在缺血期间，对照心脏和8-PT心脏的左心室舒张末压降至缺血前值的约15%，ATP和磷酸肌酸（PCr）分别降至缺血前值的约73%和60%。对照心脏和8-PT心脏中的无机磷酸盐（Pi）分别增加至缺血前值的353±41%和424±53%。再灌注后，对照心脏和8-PT心脏的功能恢复到缺血前水平的95%以上。与对照心脏不同，8-PT心脏在再灌注期间代谢物的恢复有显著差异（P<0.05）；ATP、磷酸肌酸和Pi分别恢复到缺血前值的82±8%、71±8%和281±27%。8-PT心脏在再灌注期间的静脉嘌呤清除率（327±113纳摩尔）显著高于对照心脏（127±28纳摩尔）（P<0.05）。腺苷受体的阻断似乎对缺血再灌注心肌的代谢恢复有不利影响，但对功能恢复没有影响。
