Berthoud H R, Carlson N R, Powley T L
Department of Psychological Sciences, Purdue University, West Lafayette, Indiana 47907.
Am J Physiol. 1991 Jan;260(1 Pt 2):R200-7. doi: 10.1152/ajpregu.1991.260.1.R200.
The gastrointestinal territories innervated by the gastric, celiac, and hepatic abdominal vagi were identified in rats with selective branch vagotomies by means of 1) anterograde tracing with the carbocyanine dye DiI injected into the dorsal motor nucleus and 2) measurement of cervical vagal stimulation-induced motility responses throughout the gut axis. Presence of DiI-labeled vagal terminals in the myenteric plexus and evoked motility responses were well correlated across the sampled gastrointestinal (GI) sites. In animals with only the two gastric branches intact, the entire stomach and the most proximal duodenum showed significant motility responses and were densely innervated, having DiI-labeled vagal terminals in almost every ganglion. The hepatic branch was found to primarily innervate the duodenum, with minor projections to the distal antral stomach and the intestines. The two celiac branches were found to almost exclusively innervate the jejunum, ileum, cecum and entire colon, and, together with the other vagal branches, the duodenum. Therefore, while there is some degree of specific innervation by the abdominal vagal branches of the oral-to-anal gut axis, which could be called "viscerotopic," the considerably overlapping innervation of the duodenum does not satisfy a viscerotopy criterion and needs further functional analysis.
通过以下两种方法在选择性分支迷走神经切断术的大鼠中确定由胃、腹腔和肝腹侧迷走神经支配的胃肠道区域：1）将羰花青染料DiI注入背运动核进行顺行示踪；2）测量颈迷走神经刺激在整个肠道轴上诱发的运动反应。在采样的胃肠道（GI）部位，肌间神经丛中DiI标记的迷走神经终末的存在与诱发的运动反应密切相关。在仅保留两个胃分支完整的动物中，整个胃和最近端的十二指肠表现出明显的运动反应，并且神经支配密集，几乎每个神经节都有DiI标记的迷走神经终末。发现肝支主要支配十二指肠，对胃窦远端和肠道有少量投射。发现两个腹腔支几乎专门支配空肠、回肠、盲肠和整个结肠，并且与其他迷走神经分支一起支配十二指肠。因此，虽然从口腔到肛门的肠道轴的腹侧迷走神经分支存在一定程度的特定支配，可称为“内脏定位”，但十二指肠的大量重叠支配并不符合内脏定位标准，需要进一步进行功能分析。
