CNR-INFM-S3 National Center on nanoStructures and bioSystems at Surfaces, Via Campi 213/A, 41125 Modena, Italy.
J Phys Chem B. 2009 Dec 31;113(52):16654-9. doi: 10.1021/jp907505m.
We report an atomic force microscopy study on the lateral spatial redistribution of an integral membrane protein reconstituted in supported lipid bilayers (SLBs) subjected to a thermally induced phase transition. KcsA proteins were reconstituted in proteoliposomes of POPE/POPG (3:1, mol/mol), and SLBs, including the proteins, were then obtained by the vesicle fusion technique on mica. By decreasing the temperature, the lipid bilayer passed from a liquid disordered (l(d)) phase in which the proteins are homogeneously distributed to a coexistence of solid ordered (s(o)) and l(d) domains with the proteins preferentially distributed in the l(d) domains. The inhomogeneous distribution eventually led to protein clustering. The obtained results are discussed in light of the role that the lipid/protein interaction can have in determining the function of integral membrane proteins.
我们报告了一项原子力显微镜研究，研究了在热诱导相转变下，重组在支撑脂质双层（SLB）中的整合膜蛋白的横向空间再分布。KcsA 蛋白在 POPE/POPG（3:1，摩尔比）的脂蛋白体中进行重组，然后通过囊泡融合技术在云母上获得包含蛋白的 SLB。通过降低温度，脂质双层从蛋白质均匀分布的无序液体（l(d)）相转变为固体有序（s(o)）和 l(d)相共存的相，蛋白质优先分布在 l(d)相中。不均匀的分布最终导致蛋白质聚集。所得到的结果将根据脂质/蛋白质相互作用在决定整合膜蛋白功能方面的作用进行讨论。
