Faculty of Exact and Natural Sciences, Department of Biology, I. Javakhishvili Tbilisi State University, Georgia. 2, University Str., 0143 Tbilisi, Republic of Georgia.
Scand J Clin Lab Invest. 2010 Feb;70(1):33-9. doi: 10.3109/00365510903427583.
Galactose-specific lectins (Gal-lectins) were isolated from the mitochondrial fraction of prostate post-operational hyperplasic tissue of two diagnoses: benign prostate hyperplasic tissue with low-grade intraepithelial neoplasia (LGPIN) and benign prostate hyperplasic tissue with atypical adenomatous hyperplasia (AAH). They had similar molecular weight and other properties. Effects of these lectins were investigated in vitro model experiments on bovine liver cells mitochondrial properties. Time-dependent changes: (i) in the amount of H(2)O(2); (ii) redox state of Cu in cytochrome oxidase and (iii) redox state of heme in cytochrome a+a(3) (cyt a+a(3)) of cytochrome c oxidase complex were studied. Gal-lectins from both sources increase the amount of H(2)O(2) and decrease the redox state of Cu in cytochrome oxidase and heme in cyt a+a(3). However the Gal-lectin from tissue with more severed transformation (AAH) expresses significantly more strong and long-lasting influence. These effects are mediated by galactose binding domain of the lectins as are completely abolished by the inclusion of galactose in reaction medium. Accumulation of H(2)O(2) and long-lasting decrease in the redox state of key enzymes of mitochondrial respiration chain could induce defective functioning of these organelles and whole cells. Obtained data point the possible way, which enhances further transformation of prostate tissue by release of Gal-lectins from damaged mitochondria.
半乳糖特异性凝集素（Gal-lectins）从前列腺术后增生组织的线粒体部分被分离出来，这些组织有两种诊断结果：低级别上皮内瘤变（LGPIN）和非典型腺瘤性增生（AAH）的良性前列腺增生组织。它们具有相似的分子量和其他特性。在体外牛肝细胞线粒体特性模型实验中研究了这些凝集素的作用。研究了时间依赖性变化：（i）H2O2 的量；（ii）细胞色素氧化酶中 Cu 的氧化还原状态；（iii）细胞色素 c 氧化酶复合物中细胞色素 a+a（3）（cyt a+a（3））中的血红素的氧化还原状态。两种来源的 Gal-lectins 都增加了 H2O2 的量，降低了细胞色素氧化酶中 Cu 的氧化还原状态和细胞色素 a+a（3）中的血红素的氧化还原状态。然而，来自转化程度更高（AAH）的组织的 Gal-lectin 表达出更强烈和持久的影响。这些作用是通过凝集素的半乳糖结合域介导的，而当半乳糖被包含在反应介质中时，这些作用会完全被消除。H2O2 的积累和线粒体呼吸链关键酶氧化还原状态的持久降低可能导致这些细胞器和整个细胞的功能缺陷。获得的数据指出了一种可能的途径，即通过受损线粒体释放 Gal-lectins 来增强前列腺组织的进一步转化。
