Department of Chemical Engineering, Indian Institute of Technology Roorkee, Uttarakhand, India.
Crit Rev Biotechnol. 2009;29(4):255-78. doi: 10.3109/07388550903062314.
Shaking bioreactors are the most frequently used reaction vessels in biotechnology. Since their inception, shaking bioreactors have been playing a significant role in medicine, agriculture, food, environmental, and industrial research. In spite of their huge practical importance, very little is known about the characteristic properties of shaken cultures from an engineering point of view. In this paper, a critical analysis is presented of the mixing characteristics, aeration, mass and heat transfer, power consumption, and suitability for on-line monitoring and control of various environmental and other operating parameters in aerated and anaerobic/anoxic conditions. Aspects of cell damage due to shear stress generated in shaken flask and loss of sterility due to contamination are also discussed.
摇床生物反应器是生物技术中最常用的反应容器。自问世以来，摇床生物反应器在医学、农业、食品、环境和工业研究中发挥了重要作用。尽管它们具有巨大的实际重要性，但从工程角度来看，对于摇瓶培养物的特性却知之甚少。本文从工程角度对通气和厌氧/缺氧条件下的混合特性、通气、质量和传热、功率消耗以及在线监测和控制各种环境和其他操作参数的适用性进行了分析。还讨论了由于摇瓶中产生的剪切应力而导致的细胞损伤以及由于污染而导致的无菌性丧失等方面的问题。
