Pharmacy School, Wenzhou Medical College, Zhejiang Province, China.
J Drug Target. 2010 Jul;18(6):430-7. doi: 10.3109/10611860903434043.
The objective of this study was to investigate the factors for enhancing the susceptibility of cancer cells to chemotherapeutic drug by ultrasound microbubbles. Ultrasound (US) combined with phospholipid-based microbubbles (MB) was used to enhance the susceptibility of colon cancer cell line SWD-620 to anticancer drugs Topotecan hydrochloride (TOP). Experiments were designed to investigate the influence of main factors on cell viability and cell inhibition, such as US intensity, MB concentration, drug combination with MB, asynchronous action between US triggered cavitation and drug entering cell, MB particle size. US exposure for 10 sec with US probe power at 0.6 W/cm(2) had satisfied cell viability. Treated with US combined with 15% MB, cell viability maintained more than 85% and cell inhibition 86.16%. Under optimal US combined with MB, TOP showed much higher cell inhibition than that of only TOP group. Cell inhibition under short delayed time (<2 h) for TOP addition did not show obvious difference. In terms of MB particle size, the order of cell inhibition was: Mixture > Micron bubble part > Nanometer bubble part. US combined with MB can enhance the susceptibility of cancer cells to chemotherapeutic drug, which may provide a potential method for US-mediated tumor chemotherapy.
本研究旨在探讨超声微泡增强癌细胞对化疗药物敏感性的相关因素。采用超声（US）联合磷脂基微泡（MB）增强盐酸拓扑替康（TOP）对结肠癌 SWD-620 细胞系的敏感性。实验设计主要考察了各主要因素（包括超声强度、MB 浓度、MB 与药物联合应用、US 触发空化与药物进入细胞的时间异步性、MB 粒径）对细胞活力和细胞抑制的影响。US 探头功率 0.6 W/cm2 辐照 10s 可满足细胞活力要求。用 15%MB 处理后，细胞活力保持在 85%以上，细胞抑制率为 86.16%。在最佳的超声联合 MB 条件下，与单独 TOP 组相比，TOP 显示出更高的细胞抑制率。在较短的 TOP 加入延迟时间（<2h）下，细胞抑制率没有明显差异。就 MB 粒径而言，细胞抑制率的顺序为：混合物>微米气泡部分>纳米气泡部分。超声联合 MB 可以增强癌细胞对化疗药物的敏感性，为超声介导的肿瘤化疗提供了一种潜在的方法。
