Pharmacy School, Wenzhou Medical College, Zhejiang Province 325035, China.
J Drug Target. 2011 Feb;19(2):154-60. doi: 10.3109/10611861003801834. Epub 2010 Apr 30.
An in vivo study on enhancing epirubicin hydrochloride (EPI) inhibition on tumor growth by ultrasound (US) was reported. Five-week-old male nude mice were used and HL-60 cells were s.c. (subcutaneous injection) inoculated in axilla of these mice. Six groups were designed and five consecutive treatments were applied to investigate the inhibition on tumor growth and body weight growth. US applied locally to the tumor resulted in a substantially increased drug uptake in tumor cells. The inhibition on tumor growth depended on the position of drug injection and phospholipid-based microbubble (PMB) application. Tumor growth rate under group 1 (PMB+US) was similar to that of blank control. The order of the inhibition on tumor volume growth was: group 4 (s.c. EPI+PMB+US) > group 5 intraperitoneal (i.p. EPI+PMB+US) > group 2 (i.p. EPI) > group 3 (s.c. EPI+US) > group 1 (PMB+US). Similar conclusion was obtained from experimental measurements of tumor weight change. The order of animal survival status for EPI administration groups was: group 4 > group 5 > group 2 > group 3. Chemotherapeutic drug inhibition on tumor growth could be enhanced by local US combined with PMB, which might provide a potential application for US-mediated chemotherapy.
一项关于超声（US）增强盐酸表柔比星（EPI）抑制肿瘤生长的体内研究报告如下。使用 5 周龄雄性裸鼠，将 HL-60 细胞皮下（SC）接种于这些小鼠的腋窝。设计了 6 组，进行了 5 次连续治疗，以研究对肿瘤生长和体重增长的抑制作用。局部应用 US 可使肿瘤细胞内药物摄取显著增加。肿瘤生长的抑制作用取决于药物注射部位和磷脂基微泡（PMB）的应用。组 1（PMB+US）的肿瘤生长率与空白对照组相似。肿瘤体积生长抑制率的顺序为：组 4（SC EPI+PMB+US）>组 5（腹腔内（IP）EPI+PMB+US）>组 2（IP EPI）>组 3（SC EPI+US）>组 1（PMB+US）。从肿瘤重量变化的实验测量中也得出了类似的结论。EPI 给药组动物的存活状态顺序为：组 4>组 5>组 2>组 3。局部 US 联合 PMB 可增强化疗药物对肿瘤生长的抑制作用，为 US 介导的化疗提供了一种潜在的应用。
