Elliott Gary S
Department of High Throughput Screening Molecular Pharmacology, Amgen Inc., One Amgen Center Dr, Thousand Oaks, CA 91320, USA.
Comb Chem High Throughput Screen. 2009 Nov;12(9):849-59. doi: 10.2174/138620709789383204.
As technologies for high throughput and high content screening continue to evolve, new platforms for quantitative cellular imaging will play an increasingly important role in identifying and profiling lead compounds. To gain insight into the effects of a compound on cell morphology or intracellular events, it is necessary to have quality images and the ability to enumerate thousands of data points for statistical relevance. Imaging flow cytometry combines many of the features of flow cytometry, microscopy and imaging as well as a number of unique characteristics. The result is an instrument capable of highly quantitative analysis of cellular behaviors such as receptor internalization, phagocytosis, cell-cell communication, apoptosis and nuclear translocation. This promising new technology and unique type of flow cytometry provides enhanced capabilities for highly multiplexed assays. Here, we review the capabilities of the ImageStream imaging cytometer and discuss several applications relevant to compound screening and profiling.
随着高通量和高内涵筛选技术不断发展，用于定量细胞成像的新平台在识别和分析先导化合物方面将发挥越来越重要的作用。为深入了解化合物对细胞形态或细胞内事件的影响，需要有高质量的图像以及对数千个数据点进行计数以确保统计相关性的能力。成像流式细胞术结合了流式细胞术、显微镜和成像的许多特点以及一些独特特性。其结果是一种能够对诸如受体内化、吞噬作用、细胞间通讯、细胞凋亡和核转位等细胞行为进行高度定量分析的仪器。这项前景广阔的新技术以及独特类型的流式细胞术为高度多重分析提供了增强的能力。在此，我们综述了ImageStream成像细胞仪的功能，并讨论了与化合物筛选和分析相关的几种应用。
