Zweig M H, Csako G, Reynolds J C, Carrasquillo J A
Department of Clinical Pathology, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Md. 20892.
Arch Pathol Lab Med. 1991 Feb;115(2):164-8.
Two-site immunometric assays using mouse monoclonal antibodies are gaining increasingly widespread popularity and use. Patients with circulating antimurine immunoglobulin antibodies capable of interfering in these assays have been encountered and described sporadically. Parenteral administration of murine monoclonal antibodies for imaging and therapeutic purposes is increasing and is known to induce human anti-murine antibodies frequently. We examined 60 serum samples from 48 individuals who received such murine immunoglobulin to determine whether iatrogenically induced anti-murine antibodies could interfere in a two-site (sandwich) immunoradiometric assay for serum thyrotropin. We found that these circulating antibodies can indeed interfere in an "unblocked" assay, but that the interference appears to be suppressed by including nonspecific IgG in the commercial version of the assay kit.
使用小鼠单克隆抗体的双位点免疫测定法越来越广泛地受到欢迎和应用。偶尔会遇到并描述那些体内存在能够干扰这些测定的抗鼠免疫球蛋白抗体的患者。用于成像和治疗目的的鼠单克隆抗体的肠胃外给药正在增加，并且已知其经常诱导人抗鼠抗体。我们检测了48名接受此类鼠免疫球蛋白的个体的60份血清样本，以确定医源性诱导的抗鼠抗体是否会干扰用于血清促甲状腺激素的双位点（夹心）免疫放射测定法。我们发现这些循环抗体确实会干扰“未封闭”的测定，但通过在测定试剂盒的商业版本中加入非特异性IgG，这种干扰似乎会受到抑制。
