McCarthy R C, Ryan F J, McKenzie C M
Department of Pathology Children's Hospital, Denver.
Arch Pathol Lab Med. 1988 Sep;112(9):901-7.
Serum samples from a female patient gave falsely elevated results in nine of ten immunoenzymometric assays (IEMAs) that used mouse monoclonal antibodies specific for human chorionic gonadotropin (n = 8), thyrotropin, or creatine kinase-MB isozyme. In contrast, normal results were obtained in five radioimmunoassays that used either mouse monoclonal antibodies or antisera specific for human chorionic gonadotropin (n = 3) or thyrotropin (n = 2). Incubation of her serum with IgG from different species or with F(ab)'2 from mouse IgG prior to IEMA showed that the interference was markedly inhibited by mouse IgG, indicating an antibody specific for the Fc portion of mouse IgG. The interfering activity was bound to and eluted from a column containing Protein A-Sepharose CL-4B. Fractionation of the eluted protein over another column containing Sephacryl S300 showed the activity was enriched in the first protein peak, which contained predominantly IgM. A model is proposed to explain how IgM anti-mouse IgG antibody selectively interferes in IEMAs that use mouse monoclonal antibodies.
一名女性患者的血清样本在十种免疫酶标分析（IEMA）中的九种中给出了错误的升高结果，这些分析使用了针对人绒毛膜促性腺激素（n = 8）、促甲状腺激素或肌酸激酶-MB同工酶的小鼠单克隆抗体。相比之下，在五种放射免疫分析中获得了正常结果，这些分析使用了针对人绒毛膜促性腺激素（n = 3）或促甲状腺激素（n = 2）的小鼠单克隆抗体或抗血清。在IEMA之前，将她的血清与来自不同物种的IgG或小鼠IgG的F(ab)'2孵育，结果表明该干扰被小鼠IgG显著抑制，这表明存在一种针对小鼠IgG Fc部分的特异性抗体。该干扰活性与含有蛋白A-琼脂糖CL-4B的柱子结合并从该柱子上洗脱下来。将洗脱的蛋白质在另一个含有Sephacryl S300的柱子上进行分级分离，结果表明该活性在第一个蛋白质峰中富集，该峰主要包含IgM。提出了一个模型来解释IgM抗小鼠IgG抗体如何选择性地干扰使用小鼠单克隆抗体的IEMA。
