Laboratoire de Toxicologie, Faculté de Pharmacie, Université Paris Descartes, Paris, France.
Institut de la Vision, Paris, France.
Nutr Metab (Lond). 2009 Nov 23;6:48. doi: 10.1186/1743-7075-6-48.
Olive oil and fish oils are known to possess beneficial properties for human health. We investigated whether different oils and fatty acids alone were able to decrease oxidative stress induced on corneal cells.
In our in vivo study, rats were fed with marine oils rich in polyunsaturated fatty acids (PUFA) or refined olive oil during 28 days. At the end of the protocol, corneas were analysed for their fatty acids composition to study the incorporation of fatty acids in cell membranes. In our in vitro study, a human corneal cell line was incubated with marine oils or refined olive oil and subjected to oxidative stress (tBHP 50 muM, 1 hour). Effects on reactive oxygen species generation, mitochondria and caveolin-1 expression were studied using microcytofluorometry, flow cytometry and confocal microscopy.
Our results indicate that dietary oils changed the fatty acids composition of corneal cell membranes. According to our results, PUFA-rich oils and refined olive oil (free of antioxidants) blocked reactive oxygen species production. Oleic acid, the major fatty acid of olive oil, also decreased oxidative stress. Moreover, oleic acid modified caveolin-1 expression. Antioxidant properties of oleic acid could be due to disruption of membrane microdomains such as caveolae.
Oleic acid, a potential potent modulator of oxidative stress, could be added to PUFA-rich oils to prevent oxidative stress-linked corneal pathology.
橄榄油和鱼油被认为对人体健康有益。我们研究了单独的不同油脂和脂肪酸是否能够减少诱导的角膜细胞氧化应激。
在我们的体内研究中，大鼠在 28 天内食用富含多不饱和脂肪酸（PUFA）的海洋油或精炼橄榄油。在方案结束时，分析角膜的脂肪酸组成，以研究脂肪酸在细胞膜中的掺入情况。在我们的体外研究中，用人角膜细胞系与海洋油或精炼橄榄油孵育，并进行氧化应激（tBHP50μM，1 小时）。使用微细胞荧光计、流式细胞术和共聚焦显微镜研究了对活性氧物种生成、线粒体和 caveolin-1 表达的影响。
我们的结果表明，膳食油改变了角膜细胞膜的脂肪酸组成。根据我们的结果，富含多不饱和脂肪酸的油和不含抗氧化剂的精炼橄榄油阻断了活性氧的产生。橄榄油中的主要脂肪酸油酸也降低了氧化应激。此外，油酸改变了 caveolin-1 的表达。油酸的抗氧化特性可能是由于破坏了膜微区，如 caveolae。
油酸作为一种潜在的氧化应激调节剂，可添加到富含多不饱和脂肪酸的油中，以预防与氧化应激相关的角膜病变。
