Division of Veterinary Biotechnology, National Veterinary Research and Quarantine Service, 480, Anyang 6-dong, Anyang, Gyeonggido, 430-824 South Korea.
Parasitology. 2010 Feb;137(2):241-9. doi: 10.1017/S0031182009991090. Epub 2009 Nov 23.
To investigate the phylogeny of benign Theileria parasites, we determined the complete major piroplasm surface protein (MPSP) gene sequences for 6 benign theilerial organisms, including the first from tick. Sequences were analysed alongside published sequences for 39 benign Theileria parasites, using Bayesian inference and maximum parsimony. All MPSP sequences were 852 nucleotides, except for Gansu, Wuchangbuf, VB01, and VB01; Gansu contained 873 nucleotides, and the other 3 had 855. Deduced amino acid sequences contained 284 residues, except for Gansu (291) and Wuchangbuf, VB01, and VB01 (285 each). Pairwise comparisons showed identities among 45 theilerial MPSP sequences ranging from 70.9 to 99.8% for nucleotide and 71.0 to 100% for amino acid sequences. Our results clearly indicate that all global parasites, excluding Brisbane, were classified into 1 of 8 types; 6 types of Theileria exist in Korea. Each type, excluding Type 6, has several type-specific amino acid sequences. The phylogenetic tree derived from the nucleotide sequences showed 2 sister-group relationships, Type 2+Type 7 and Type 3+Brisbane, with a new branching pattern: (Type 6 (Type 8 ((Type 2, Type 7), (Type 1, (Type 4, (Type 5, (Type 3, Brisbane))))))). Our sequence data showed no geographical influence on worldwide Theileria parasite distribution.
为了研究良性泰勒虫寄生虫的系统发育，我们确定了 6 种良性泰勒虫生物体的完整主要血孢子蛋白（MPSP）基因序列，其中包括首次从蜱虫中获得的序列。使用贝叶斯推断和最大简约法，对这些序列与 39 种已发表的良性泰勒虫寄生虫序列进行了分析。所有 MPSP 序列均为 852 个核苷酸，除了甘肃株、武昌株、VB01 株和 VB01 株；甘肃株含有 873 个核苷酸，其他 3 株分别含有 855 个核苷酸。推断出的氨基酸序列含有 284 个残基，除了甘肃株（291 个）和武昌株、VB01 株和 VB01 株（各 285 个）。成对比较显示，在 45 种泰勒氏 MPSP 序列之间，核苷酸序列的同一性范围为 70.9%至 99.8%，氨基酸序列的同一性范围为 71.0%至 100%。我们的结果清楚地表明，所有全球寄生虫，除了 Brisbane 株，都被分为 1 个 8 型之一；韩国存在 6 种 Theileria 型。除了 6 型之外，每种类型都有几个特定于类型的氨基酸序列。从核苷酸序列得出的系统发育树显示出 2 个姐妹群关系，即 2+7 型和 3+Brisbane 型，具有一个新的分支模式：（6 型（8 型（2+7 型，（1 型，（4 型，（5 型，（3+Brisbane 型）））））。我们的序列数据表明，全球范围内的泰勒虫寄生虫分布没有受到地理位置的影响。
