Dyson Melissa C, Eaton Kathryn A, Chang Cherie
Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan, USA.
J Am Assoc Lab Anim Sci. 2009 Nov;48(6):754-6.
Wild rodents are a potential source for pathogen introduction into laboratory animal research facilities. A study was designed to assess wild mice found at our institution by infectious disease surveillance. Wild white-footed mice (Peromyscus leucopus) were captured with live capture traps placed in areas in which wild mice had been reported in several animal facilities. Captured animals were euthanized by inhalation of CO(2), blood was collected by cardiocentesis (n = 10), and necropsy was performed (n = 8). Serum samples were negative for antibodies to mouse parvovirus (types 1 and 2), mouse minute virus, Sendai virus, pneumonia virus of mice, mouse hepatitis virus, Theiler murine encephalomyelitis virus, reovirus, rotavirus, lymphocytic choriomeningitis virus, mouse adenovirus, ectromelia virus, K virus, cilia-associated respiratory bacillus, and Mycoplasma pulmonis. Of the 8 animals that were necropsied, pelt and cecal examinations were negative for ectoparasites and pinworms, respectively. Histopathologic examination of brain, heart, lungs, liver, kidney, spleen, stomach, and small intestine revealed bacteria morphologically compatible with Helicobacter spp. in the cecal and colonic glands and occasionally in the gastric lumen and pits. Mesenteric lymph nodes and feces from 8 of the animals were submitted for PCR analysis for the detection of mouse parvovirus, mouse minute virus, mouse hepatitis virus, and Helicobacter spp.; 7 of the samples were PCR-positive for Helicobacter spp. At this time, wild mice found in our animal facilities do not appear to be a significant source of common laboratory mouse viral pathogens. However, they are a potential source of Helicobacter infections.
野生啮齿动物是将病原体引入实验动物研究设施的潜在来源。本研究旨在通过传染病监测评估在我们机构发现的野生小鼠。野生白足鼠（白足鼠属）是用活捕陷阱捕获的，这些陷阱放置在几个动物设施中曾报告有野生小鼠的区域。捕获的动物通过吸入二氧化碳实施安乐死，通过心脏穿刺采集血液（n = 10），并进行尸检（n = 8）。血清样本检测小鼠细小病毒（1型和2型）、小鼠微小病毒、仙台病毒、小鼠肺炎病毒、小鼠肝炎病毒、泰勒鼠脑脊髓炎病毒、呼肠孤病毒、轮状病毒、淋巴细胞性脉络丛脑膜炎病毒、小鼠腺病毒、鼠痘病毒、K病毒、纤毛相关呼吸道杆菌和肺支原体抗体均为阴性。在接受尸检的8只动物中，皮张和盲肠检查分别未发现体外寄生虫和蛲虫。对脑、心、肺、肝、肾、脾、胃和小肠进行组织病理学检查，结果显示在盲肠和结肠腺中，偶尔在胃腔和胃小凹中发现形态上与螺杆菌属相符的细菌。将8只动物的肠系膜淋巴结和粪便送去进行聚合酶链反应（PCR）分析，以检测小鼠细小病毒、小鼠微小病毒、小鼠肝炎病毒和螺杆菌属；7份样本PCR检测螺杆菌属呈阳性。目前，在我们动物设施中发现的野生小鼠似乎不是常见实验小鼠病毒病原体的重要来源。然而，它们是螺杆菌感染的潜在来源。
