Gong Y, Li Enyou, Xu G, Wang H, Wang C, Li P, He Y
Department of Anaesthesiology, The First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang, China.
J Int Med Res. 2009 Sep-Oct;37(5):1465-71. doi: 10.1177/147323000903700522.
Propofol has been detected in human breath after being used as an intravenous anaesthetic, and this could provide a noninvasive method for monitoring propofol anaesthesia. The physicochemical properties of propofol allow it to diffuse across the alveolocapillary membrane and to be prepared as a calibration gas. In this study, headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS), coupled with an external standard, was applied to assess propofol levels in the breath and plasma from three subjects under intravenous anaesthesia. Lower quantitation limits were 3.6 ng/l and 0.2 mg/l for propofol analysis in breath and arterial plasma, respectively. Intraday precision and recovery percentages for propofol detection in breath were 4.3 - 6.7% and 98 - 108%, respectively, and in plasma they were 3.8 - 6.1% and 90.1 - 125.1%, respectively. Propofol concentrations were 4.3 - 33.5 ng/l in breath and 3.2 - 6.8 mg/l in arterial plasma. A correlation was shown between propofol concentration in breath and plasma. Thus, HS-SPME-GC-MS, coupled with an external standard, could be a reliable and sensitive analytical technique for detecting propofol in breath during anaesthesia.
异丙酚作为静脉麻醉剂使用后已在人体呼出气体中被检测到，这可为监测异丙酚麻醉提供一种非侵入性方法。异丙酚的物理化学性质使其能够扩散穿过肺泡毛细血管膜，并可制备成校准气体。在本研究中，顶空固相微萃取气相色谱 - 质谱联用技术（HS - SPME - GC - MS）结合外标法，用于评估三名接受静脉麻醉的受试者呼出气体和血浆中的异丙酚水平。呼出气体和动脉血浆中异丙酚分析的较低定量限分别为3.6 ng/l和0.2 mg/l。呼出气体中异丙酚检测的日内精密度和回收率分别为4.3 - 6.7%和98 - 108%，血浆中的分别为3.8 - 6.1%和90.1 - 125.1%。呼出气体中异丙酚浓度为4.3 - 33.5 ng/l，动脉血浆中为3.2 - 6.8 mg/l。呼出气体和血浆中的异丙酚浓度之间显示出相关性。因此，HS - SPME - GC - MS结合外标法可能是一种可靠且灵敏的分析技术，用于在麻醉期间检测呼出气体中的异丙酚。
