Lu Yan, Niu Wenqi, Zou Xue, Shen Chengyin, Xia Lei, Huang Chaoqun, Wang Hongzhi, Jiang Haihe, Chu Yannan
Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, No. 350 Shushanhu Road, Hefei 230031, China; Anhui Province Key Laboratory of Medical Physics and Technology, No. 350 Shushanhu Road, Hefei 230031, China.
School of Science, Anhui Agricultural University, No. 130 West Changjiang Road, Hefei 230036, China.
J Chromatogr A. 2017 May 5;1496:20-24. doi: 10.1016/j.chroma.2017.03.061. Epub 2017 Mar 23.
Breath analysis is a non-invasive approach which may be applied to disease diagnosis and pharmacokinetic study. In the case of offline analysis, the exhaled gas needs to be collected and the sampling bag is often used as the storage vessel. However, the sampling bag usually releases some extra compounds, which may interfere with the result of the breath test. In this study, a novel breath sampling glass bottle was developed with a syringe needle sampling port for solid phase microextraction (SPME). Such a glass bottle scarcely liberates compounds and can be used to collect exhaled gas for ensuing analysis by gas chromatography-mass spectrometry (GC-MS). The glass bottle sampling SPME-GC-MS analysis was carried out to investigate the breath metabolites of myrtol, a multicompound drug normally used in the treatment of bronchitis and sinusitis. Four compounds, α-pinene, 2,3-dehydro-1,8-cineole, d-limonene and 1,8-cineole were found in the exhaled breath of all eight volunteers who had taken the myrtol. While for other ten subjects who had not used the myrtol, these compounds were undetectable. In the SPME-GC-MS analysis of the headspace of myrtol, three compounds were detected including α-pinene, d-limonene and 1,8-cineole. Comparing the results of breath and headspace analysis, it indicates that 2,3-dehydro-1,8-cineole in the breath is the metabolite of 1,8-cineole. It is the first time that this metabolite was identified in human breath. The study demonstrates that the glass bottle sampling SPME-GC-MS method is applicable to exhaled gas analysis including breath metabolites investigation of drugs like myrtol.
呼气分析是一种可应用于疾病诊断和药代动力学研究的非侵入性方法。在离线分析的情况下，需要收集呼出气体，采样袋常被用作储存容器。然而，采样袋通常会释放一些额外的化合物，这可能会干扰呼气测试的结果。在本研究中，开发了一种新型的呼气采样玻璃瓶，其带有用于固相微萃取（SPME）的注射器针头采样口。这种玻璃瓶几乎不释放化合物，可用于收集呼出气体，以便随后通过气相色谱 - 质谱联用仪（GC - MS）进行分析。采用玻璃瓶采样SPME - GC - MS分析方法来研究桃金娘油（一种常用于治疗支气管炎和鼻窦炎的多成分药物）的呼气代谢产物。在所有八名服用了桃金娘油的志愿者的呼出气体中发现了四种化合物，即α - 蒎烯、2,3 - 脱氢 - 1,8 - 桉叶素、d - 柠檬烯和1,8 - 桉叶素。而对于其他十名未使用桃金娘油的受试者，这些化合物未被检测到。在桃金娘油顶空的SPME - GC - MS分析中，检测到了三种化合物，包括α - 蒎烯、d - 柠檬烯和1,8 - 桉叶素。比较呼气和顶空分析的结果表明，呼气中的2,3 - 脱氢 - 1,8 - 桉叶素是1,8 - 桉叶素的代谢产物。这是首次在人体呼气中鉴定出这种代谢产物。该研究表明，玻璃瓶采样SPME - GC - MS方法适用于呼出气体分析，包括对桃金娘油等药物的呼气代谢产物研究。
