Mo Chen, Wang Ru-huan, Meng Xiang-fei, Long Chao-liang, Wang Hai
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009 Nov;21(11):652-5.
To investigate the therapeutic effects of benthiactzine against respiratory failure induced by cholinesterase inhibitor dimethyl dichloro-vinyl phosphate (DDVP) in rats.
Forty-five male Wistar rats were divided into five groups randomly: control group, model group, and benthiactzine 0.5, 1.0, 2.0 mg/kg treatment groups (each n=9). Rats were treated with DDVP by intraperitoneal injection to reproduce respiratory failure model. The symptoms, respiratory rate (RR), blood gas analysis, electrolyte and plasma superoxide dismutase (SOD), malondialdehyde (MDA) and the pathological changes were observed before poisoning, during respiratory failure, and in different periods after the treatment.
In rats with respiratory failure induced by DDVP, cyanosis and convulsion occurred in all groups. The success rates in three benthiactzine groups were 66.7% (6/9), 77.8% (7/9) and 88.9% (8/9). The rats of benthiactzine treatment groups recovered in 1-5 minutes after treatment and returned to normal state in 30 minutes. RR also returned to normal in 30 minutes. When respiratory failure occurred, arterial oxygen partial pressure (PaO2), arterial oxygen saturation (SaO2) and plasma SOD were decreased, plasma MDA was increased, and mixed acidosis was found. Thirty minutes after the treatment of benthiactzine, all above parameters in three groups returned to normal (all P<0.01). In respiratory failure rats, pathological examination of lung tissue revealed dilatation of pulmonary vessels with aggregation of erythrocytes, widening of alveolar space with presence of red blood cells in alveoli with heavy infiltration of inflammatory cells, and pulmonary edema and hemorrhage. The lungs of rats treated with benthiactzine showed less intense pathological changes.
The new medicine against poisoning benthiactzine can be a favourable drug against respiratory failure induced by organophosphorus pesticides.
探讨苯噻嗪对大鼠有机磷农药敌敌畏所致呼吸衰竭的治疗作用。
45只雄性Wistar大鼠随机分为5组：对照组、模型组、苯噻嗪0.5、1.0、2.0mg/kg治疗组（每组n = 9）。通过腹腔注射敌敌畏使大鼠建立呼吸衰竭模型。观察中毒前、呼吸衰竭时及治疗后不同时期的症状、呼吸频率（RR）、血气分析、电解质、血浆超氧化物歧化酶（SOD）、丙二醛（MDA）及病理变化。
敌敌畏致呼吸衰竭大鼠各模型组均出现发绀、抽搐。苯噻嗪3个治疗组的成功率分别为66.7%（6/9）、77.8%（7/9）和88.9%（8/9）。苯噻嗪治疗组大鼠给药后1 - 5分钟苏醒，30分钟恢复正常状态。RR在30分钟时也恢复正常。呼吸衰竭发生时，动脉血氧分压（PaO2）、动脉血氧饱和度（SaO2）及血浆SOD降低，血浆MDA升高，出现混合性酸中毒。苯噻嗪治疗30分钟后，3组上述指标均恢复正常（均P<0.01）。呼吸衰竭大鼠肺组织病理检查显示肺血管扩张，红细胞聚集，肺泡腔增宽，肺泡内有红细胞，炎症细胞重度浸润，伴肺水肿和出血。苯噻嗪治疗组大鼠肺组织病理改变较轻。
新型抗中毒药物苯噻嗪可成为治疗有机磷农药所致呼吸衰竭的有效药物。
