Department of Emergency Medicine, Kayseri Training and Research Hospital, Kayseri, Turkey.
Biol Trace Elem Res. 2010 Feb;133(2):227-35. doi: 10.1007/s12011-009-8418-6. Epub 2009 Jun 6.
Although atropine and oximes are traditionally used in the management of organophosphate poisoning, investigations have been directed to finding additional therapeutic approaches. Thus, the aim of this study was to evaluate the cardiac effects of magnesium sulfate pretreatment on dichlorvos intoxication in rats. Rats were randomly divided into three groups as control, dichlorvos, and magnesium sulfate groups. After 6 h of dichlorvos or corn oil (as a vehicle) injection, venous blood samples were collected, and cardiac tissue samples were obtained. Biochemical analyses were performed to measure some parameters on serum and cardiac tissue. Immunohistochemical analyses of apoptosis and inducible nitric oxide (NO) synthase showed no change in cardiac tissue. Serum cholinesterase levels were markedly depressed with dichlorvos, and further suppressed markedly with magnesium sulfate pretreatment. Although we have demonstrated that serum NO levels in dichlorvos and magnesium sulfate groups were lower than the control group, cardiac tissue NO levels in magnesium sulfate group were higher than the other two groups. Mortality was not significantly affected with magnesium sulfate pretreatment. Uncertainty still persists on the right strategies for the treatment of organophosphate acute poisoning; however, it was concluded that our results do not suggest that magnesium sulfate therapy is beneficial in the management of acute dichlorvos-induced organophosphate poisoning, and also further studies are required.
尽管阿托品和肟类药物传统上用于有机磷中毒的治疗，但研究一直致力于寻找其他治疗方法。因此，本研究旨在评估硫酸镁预处理对大鼠敌敌畏中毒的心脏效应。大鼠随机分为三组：对照组、敌敌畏组和硫酸镁组。敌敌畏或玉米油（作为载体）注射 6 小时后，采集静脉血样和心脏组织样本。进行生化分析以测量血清和心脏组织中的一些参数。凋亡和诱导型一氧化氮合酶的免疫组织化学分析显示心脏组织无变化。血清胆碱酯酶水平在敌敌畏组中明显降低，而硫酸镁预处理进一步明显抑制。尽管我们已经证明敌敌畏和硫酸镁组的血清 NO 水平低于对照组，但硫酸镁组的心脏组织 NO 水平高于其他两组。硫酸镁预处理对死亡率没有显著影响。对于有机磷急性中毒的治疗策略仍然存在不确定性；然而，我们的结果表明，硫酸镁治疗在管理急性敌敌畏诱导的有机磷中毒方面没有益处，还需要进一步研究。
