Qiu Hui, Dong Sheng-li, Tu Yong-jiu, Liang Hua-ping
State Key Laboratory of Trauma, Burns, and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
Chin J Traumatol. 2009 Dec;12(6):334-8.
To study the role of dendritic cells (DCs) in initiating delayed-type hypersensitivity (DTH) to fluorescein isothiocyanate (FITC) after trauma-hemorrhage in mice.
Inbred BALB/c mice (6-8 weeks old, male) were epicutaneously sensitized with FITC 12 hours, 1 day, 2 days, 4 days and 7 days after closed bilateral femur fractures combined with hemorrhage. And 5 days after sensitization, DTH was evaluated by ear swelling after a challenge of FITC. Draining lymph node cells were examined for the percentages of FITC-positive cells, cluster of differentiation (CD)11c-positive cells and major histocompatibility complex II (MHC II)-positive cells by means of flow cytometry. In vitro proliferative responses of syngeneic lymphocytes and in vivo passive transfer of DTH to naive recipients induced by isolated DCs from the draining lymph nodes were determined.
The time of DTH to FITC decreased more significantly in the mice with trauma-hemorrhage (12 hours to 4 days) than in the mice with sham injury. After sensitization, the relative percentages of FITC+ cells, FITC+/CD11c+ cells and FITC+/CD11c+/MHC II+ cells from the draining lymph nodes were all significantly reduced following injury. And the capacity of DCs from the draining lymph nodes in stimulating proliferative responses of lymphocytes and transferring DTH to naive recipients were also inhibited after injury.
Trauma-hemorrhage induces repressive DTH in mice, which may be attributed, at least partially, to the reduced trafficking of DCs into the draining lymph nodes and insufficient maturation during DC migration.
研究树突状细胞（DCs）在小鼠创伤性出血后引发对异硫氰酸荧光素（FITC）的迟发型超敏反应（DTH）中的作用。
近交系BALB/c小鼠（6 - 8周龄，雄性）在双侧股骨闭合性骨折合并出血后12小时、1天、2天、4天和7天经皮用FITC致敏。致敏后5天，通过FITC激发后的耳部肿胀评估DTH。通过流式细胞术检测引流淋巴结细胞中FITC阳性细胞、分化簇（CD）11c阳性细胞和主要组织相容性复合体II（MHC II）阳性细胞的百分比。测定同基因淋巴细胞的体外增殖反应以及由引流淋巴结中分离的DCs诱导的DTH对未致敏受体的体内被动转移。
创伤性出血小鼠（12小时至4天）对FITC的DTH时间比假损伤小鼠更显著缩短。致敏后，损伤后引流淋巴结中FITC +细胞、FITC + / CD11c +细胞和FITC + / CD11c + / MHC II +细胞的相对百分比均显著降低。损伤后，引流淋巴结中DCs刺激淋巴细胞增殖反应和将DTH转移至未致敏受体的能力也受到抑制。
创伤性出血在小鼠中诱导抑制性DTH，这可能至少部分归因于DCs向引流淋巴结的迁移减少以及DC迁移过程中成熟不足。
