Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Japan.
Comp Immunol Microbiol Infect Dis. 2011 Jan;34(1):35-9. doi: 10.1016/j.cimid.2009.10.007.
Bovine viral diarrhea virus (BVDV) field isolates show genetic and antigenic diversity. At least 14 subgenotypes of BVDV-1 and 4 of BVDV-2 have been identified in Artiodactyla worldwide. Of these, 6 subgenotypes of BVDV-1 and 1 of BVDV-2 have been isolated in Japan. Previously, we reported that each subgenotype virus expresses different antigenic characteristics. Here we investigated the reactivity of neutralizing antibodies against representative strains of Japanese BVDV subgenotypes using sera from 266 beef cattle to estimate the prevalence of this epidemic virus among cattle in Japan. Antibody titers at concentrations at least 4-fold higher than antibodies against other subgenotype viruses were considered subgenotype specific. Subgenotype-specific antibodies were detected from 117 (80.7%) of 145 sera samples (69.7% against BVDV-1a, 1.4% against BVDV-1b, 8.3% against BVDV-1c, and 1.4% against BVDV-2a). The results suggest that neutralization tests are useful in estimating currently epidemic subgenotypes of BVDV in the field.
牛病毒性腹泻病毒（BVDV）田间分离株表现出遗传和抗原多样性。在全球有蹄类动物中，已鉴定出至少 14 种 BVDV-1 亚型和 4 种 BVDV-2 亚型。其中，日本已分离出 6 种 BVDV-1 亚型和 1 种 BVDV-2 亚型。此前，我们报道过每种亚群病毒表达不同的抗原特征。在这里，我们使用来自 266 头肉牛的血清研究了日本 BVDV 亚群代表株的中和抗体反应性，以评估这种流行病毒在日本牛群中的流行情况。抗体滴度至少比针对其他亚群病毒的抗体高 4 倍被认为是亚群特异性的。从 145 份血清样本中的 117 份（80.7%）中检测到了亚群特异性抗体（69.7%针对 BVDV-1a、1.4%针对 BVDV-1b、8.3%针对 BVDV-1c 和 1.4%针对 BVDV-2a）。结果表明，中和试验可用于估计田间 BVDV 的当前流行亚群。
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